| Gene Name | PI4KB |
| HF Protein Name | Phosphatidylinositol 4-kinase beta |
| HF Function | Required for spike-mediated Cell Entry |
| Uniprot ID | Q9UBF8 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 5298 |
| Host Factor (HF) Name in Paper | PI4KB |
| Gene synonyms | PIK4CB |
| Ensemble Gene ID | ENSG00000143393 |
| Ensemble Transcript | ENST00000368872 [Q9UBF8-2];ENST00000368873 [Q9UBF8-1];ENST00000368874 [Q9UBF8-2];ENST00000529142 [Q9UBF8-3] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000139, GO:0004430, GO:0005524, GO:0005737, GO:0005741, GO:0005768, GO:0005829, GO:0006661, GO:0006898, GO:0007165, GO:0016020, GO:0030867, GO:0048015, GO:0048471, GO:0071889, |
| MINT ID | Q9UBF8 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 602758 |
| PANTHER ID | PTHR10048 |
| PDB ID(s) | 2N73, 4D0L, 4D0M, 4WAE, 4WAG, 5C46, 5C4G, 5EUQ, 5FBL, 5FBQ, 5FBR, 5FBV, 5FBW, 5LX2, 5NAS, |
| pfam ID | PF00454, |
| Drug Bank ID | N.A., |
| ChEMBL ID | CHEMBL3268 |
| Organism | Homo sapiens (Human) |
| Virus Name | Human SARS coronavirus |
| Virus Short Name | SARS-CoV |
| Order | Nidovirales |
| Virus Family | Coronaviridae |
| Virus Subfamily | Coronavirinae |
| Genus | Betacoronavirus |
| Species | Betacoronavirus 1 |
| Host | Bats, human |
| Cell Tropism | Epithelial cells of respiratory or enteric tracts, neurological tissues are also frequently infected |
| Associated Disease | Mainly respiratory diseases |
| Mode of Transmission | Respiratory or fecal-oral in humans |
| VIPR DB link | https://www.viprbrc.org/brc/vipr_allSpecies_search.spg?method=SubmitForm&decorator=corona |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/222/coronaviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Coronaviridae |
| Paper Title | Phosphatidylinositol 4-kinase IIIbeta is required for severe acute respiratory syndrome coronavirusspike-mediated cell entry |
| Author's Name | Ning Yang, Ping Ma, Jianshe Lang, Yanli Zhang, Jiejie Deng, Xiangwu Ju, Gongyi Zhang and Chengyu Jiang |
| Journal Name | JOURNAL OF BIOLOGICAL CHMISTRY |
| Pubmed ID | 22253445 |
| Abstract | Phosphatidylinositol kinases (PI kinases) play an important role in the life cycle of several viruses after infection. Using gene knockdown technology, we demonstrate that phosphatidylinositol 4-kinase IIIbeta (PI4KB) is required for cellular entry by pseudoviruses bearing the severe acute respiratory syndrome-coronavirus (SARS-CoV) spike protein and that the cell entry mediated by SARS-CoV spike protein is strongly inhibited by knockdown of PI4KB. Consistent with this observation, pharmacological inhibitors of PI4KB blocked entry of SARS pseudovirions. Further research suggested that PI4P plays an essential role in SARS-CoV spike-mediated entry, which is regulated by the PI4P lipid microenvironment. We further demonstrate that PI4KB does not affect virus entry at the SARS-CoV S-ACE2 binding interface or at the stage of virus internalization but rather at or before virus fusion. Taken together, these results indicate a new function for PI4KB and suggest a new drug target for preventing SARS-CoV infection. |
| Used Model | Vero E6 and 293T cells |
| DOI | 10.1074/jbc.M111.312561 |
