| Gene Name | MT-ND4L |
| HF Protein Name | NADH-ubiquinone oxidoreductase chain 4L |
| HF Function | Involved in Borna Disease Virus cell entry |
| Uniprot ID | P03901 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 4539 |
| Host Factor (HF) Name in Paper | MT-ND4L |
| Gene synonyms | MTND4L NADH4L ND4L |
| Ensemble Gene ID | ENSG00000212907 |
| Ensemble Transcript | ENST00000361335 |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0005747, GO:0006120, GO:0008137, GO:0016021, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 516004 |
| PANTHER ID | PTHR11434 |
| PDB ID(s) | 5XTC, 5XTD, |
| pfam ID | N.A., |
| Drug Bank ID | DB00157, |
| ChEMBL ID | CHEMBL2363065 |
| Organism | Homo sapiens (Human) |
| Virus Name | Borna disease virus |
| Virus Short Name | BDV |
| Order | Mononegavirales |
| Virus Family | Bornaviridae |
| Virus Subfamily | N.A. |
| Genus | Bornavirus |
| Species | Borna disease virus |
| Host | Horses, sheep, cattle, rodents, birds, sometimes human also |
| Cell Tropism | Neurons and astrocytes, also infect oligodendrocytes and ependymal cells |
| Associated Disease | Borna disease ( in mammals), encephalitis, proventricular dilatation disease (in birds) |
| Mode of Transmission | Direct contact with salivary, urine and feces |
| VIPR DB link | N.A. |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/195/bornaviridae |
| Virus Host DB link | N.A. |
| Paper Title | Identification of host factors involved in borna disease virus cell entry through a small interfering RNA functional genetic screen |
| Author's Name | Roberto Clemente, Eugene Sisman, Pedro Aza-Blanc and Juan C. de la Torre |
| Journal Name | Journal Of Virology |
| Pubmed ID | 20071576 |
| Abstract | Borna disease virus (BDV), the prototypic member of the Bornaviridae family, within the order Mononegavirales, is highly neurotropic and constitutes an important model system for the study of viral persistence in the central nervous system (CNS) and associated disorders. The virus surface glycoprotein (G) has been shown to direct BDV cell entry via receptor-mediated endocytosis, but the mechanisms governing cell tropism and propagation of BDV within the CNS are unknown. We developed a small interfering RNA (siRNA)-based screening to identify cellular genes and pathways that specifically contribute to BDV G-mediated cell entry. Our screen relied on silencing-mediated increased survival of cells infected with rVSVDeltaG*/BDVG, a cytolytic recombinant vesicular stomatitis virus expressing BDV G that mimics the cell tropism and entry pathway of bona fide BDV. We identified 24 cellular genes involved in BDV G-mediated cell entry. Identified genes are known to participate in a broad range of distinct cellular functions, revealing a complex process associated with BDV cell entry. The siRNA-based screening strategy we have developed should be applicable to identify cellular genes contributing to cell entry mediated by surface G proteins of other viruses. |
| Used Model | Ol cells |
| DOI | 10.1128/JVI.02274-09 |
