| Gene Name | SLAMF1 |
| HF Protein Name | Signaling lymphocytic activation molecule |
| HF Function | Required for adaptation of CDV to the human entry receptors |
| Uniprot ID | Q95MM9 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 403642 |
| Host Factor (HF) Name in Paper | CD150 |
| Gene synonyms | SLAM |
| Ensemble Gene ID | ENSG00000117090 |
| Ensemble Transcript | ENSCAFT00000019982 |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0001779, GO:0001787, GO:0002232, GO:0002725, GO:0009897, GO:0010759, GO:0016021, GO:0016032, GO:0031338, GO:0035744, GO:0038023, GO:0042104, GO:0042802, GO:0045087, GO:0045335, GO:0050790, GO:1902714, GO:1902715, GO:2000510, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | N.A. |
| PANTHER ID | N.A. |
| PDB ID(s) | N.A., |
| pfam ID | PF06214, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Canis lupus familiaris (Dog) (Canis familiaris) |
| Virus Name | Canine Distemper Virus |
| Virus Short Name | CDV |
| Order | Mononegavirales |
| Virus Family | Paramyxoviridae |
| Virus Subfamily | N.A. |
| Genus | Morbillivirus |
| Species | Canine morbillivirus |
| Host | Dogs, coyotes, foxes, pandas and larg cates |
| Cell Tropism | N.A. |
| Associated Disease | Severe pneumonia with vomiting, bloody diarrhea and death |
| Mode of Transmission | N.A. |
| VIPR DB link | http://www.viprbrc.org/brc/vipr_allSpecies_search.do?method=SubmitForm&decorator=paramyxo |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/199/paramyxoviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Paramyxoviridae |
| Paper Title | Experimental adaptation of wild Type canine distemper virus (CDV) to the human entry receptor CD150 |
| Author's Name | Maria Bieringer, Jung Woo Han, Sabine Kendl, Mojtaba Khosravi, Philippe Plattet, Ju rgen SchneiderSchaulies |
| Journal Name | PLOS One |
| Pubmed ID | 23554862 |
| Abstract | Canine distemper virus (CDV), a close relative of measles virus (MV), is widespread and well known for its broad host range. When the goal of measles eradication may be achieved, and when measles vaccination will be stopped, CDV might eventually cross the species barrier to humans and emerge as a new human pathogen. In order to get an impression how fast such alterations may occur, we characterized required adaptive mutations to the human entry receptors CD150 (SLAM) and nectin-4 as first step to infect human target cells. Recombinant wild-type CDV-A75/17(red) adapted quickly to growth in human H358 epithelial cells expressing human nectin-4. Sequencing of the viral attachment proteins (hemagglutinin, H, and fusion protein, F) genes revealed that no adaptive alteration was required to utilize human nectin-4. In contrast, the virus replicated only to low titres (10(2) pfu/ml) in Vero cells expressing human CD150 (Vero-hSLAM). After three passages using these cells virus was adapted to human CD150 and replicated to high titres (10(5) pfu/ml). Sequence analyses revealed that only one amino acid exchange in the H-protein at position 540 Asp→Gly (D540G) was required for functional adaptation to human CD150. Structural modelling suggests that the adaptive mutation D540G in H reflects the sequence alteration from canine to human CD150 at position 70 and 71 from Pro to Leu (P70L) and Gly to Glu (G71E), and compensates for the gain of a negative charge in the human CD150 molecule. Using this model system our data indicate that only a minimal alteration, in this case one adaptive mutation, is required for adaptation of CDV to the human entry receptors, and help to understand the molecular basis why this adaptive mutation occurs. |
| Used Model | NCI- H358 and Vero-hSLAM cells |
| DOI | 10.1371/journal.pone.0057488 |
