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Virus Details


VHFID177

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name BRD4
HF Protein Name Bromodomain-containing protein 4
HF Function Inhibits viral infection
Uniprot ID O60885
Protein Sequence View Fasta Sequence
NCBI Gene ID 23476
Host Factor (HF) Name in Paper BRD4
Gene synonyms HUNK1
Ensemble Gene ID ENSG00000141867
Ensemble Transcript ENST00000263377 [O60885-1];ENST00000360016 [O60885-3];ENST00000371835 [O60885-2]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000083, GO:0000794, GO:0000993, GO:0002039, GO:0003682, GO:0005634, GO:0005654, GO:0005694, GO:0005829, GO:0006325, GO:0006338, GO:0006351, GO:0006974, GO:0010971, GO:0016032, GO:0032968, GO:0043123, GO:0045944, GO:0050727, GO:0070577, GO:1901407, GO:2000002,
MINT ID O60885
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 608749
PANTHER ID N.A.
PDB ID(s) 2I8N, 2LSP, 2MJV, 2N3K, 2NCZ, 2ND0, 2ND1, 2NNU, 2OSS, 2OUO, 2YEL, 2YEM, 3MXF, 3P5O, 3SVF, 3SVG, 3U5J, 3U5K, 3U5L, 3UVW, 3UVX, 3UVY, 3UW9, 3ZYU, 4A9L, 4BJX, 4BW1, 4BW2, 4BW3, 4BW4, 4C66, 4C67, 4CFK, 4CFL, 4CL9, 4CLB, 4DON, 4E96, 4F3I, 4GPJ, 4HBV, 4HBW, 4HBX, 4HBY, 4HXK, 4HXL, 4HXM, 4HXN, 4HXO, 4HXP, 4HXR, 4HXS, 4IOO, 4IOQ, 4IOR, 4J0R, 4J0S, 4J3I, 4KV1, 4KV4, 4LR6, 4LRG, 4LYI, 4LYS, 4LYW, 4LZR, 4LZS, 4MEN, 4MEO, 4MEP, 4MEQ, 4MR3, 4MR4, 4NQM, 4NR8, 4NUC, 4NUD, 4NUE, 4O70, 4O71, 4O72, 4O74, 4O75, 4O76, 4O77, 4O78, 4O7A, 4O7B, 4O7C, 4O7E, 4O7F, 4OGI, 4OGJ, 4PCE, 4PCI, 4PS5, 4QB3, 4QR3, 4QR4, 4QR5, 4QZS, 4UIX, 4UIY, 4UIZ, 4UYD, 4WHW, 4WIV, 4X2I, 4XY9, 4XYA, 4YH3, 4YH4, 4Z1Q, 4Z1S, 4Z93, 4ZC9, 4ZW1, 5A5S, 5A85, 5ACY, 5AD2, 5AD3, 5BT4, 5CFW, 5COI, 5CP5, 5CPE, 5CQT, 5CRM, 5CRZ, 5CS8, 5CTL, 5CY9, 5D0C, 5D24, 5D25, 5D26, 5D3H, 5D3J, 5D3L, 5D3N, 5D3P, 5D3R, 5D3S, 5D3T, 5DLX, 5DLZ, 5DW2, 5DX4, 5E0R, 5EGU, 5EI4, 5EIS, 5F5Z, 5F60, 5F61, 5F62, 5F63, 5FBX, 5H21, 5HCL, 5HLS, 5HM0, 5HQ5, 5HQ6, 5HQ7, 5I80, 5I88, 5IGK, 5JWM, 5KDH, 5KHM, 5KJ0, 5KU3, 5LJ1, 5LJ2, 5LRQ, 5LUU, 5M39, 5M3A, 5MKZ, 5MLI, 5N2M, 5T35, 5TI2, 5TI3, 5TI4, 5TI5, 5TI6, 5TI7, 5U28, 5U2C, 5U2E, 5U2F, 5UEO, 5UEP, 5UEQ, 5UER, 5UES, 5UET, ,
pfam ID PF17035, PF17105, PF00439,
Drug Bank ID N.A.,
ChEMBL ID CHEMBL1163125
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Adeno-associated virus 2
Virus Short Name AAV2
Order Unassigned
Virus Family Parvoviridae
Virus Subfamily Parvovirinae
Genus Dependovirus
Species Adeno-associated virus 2
Host Human, vertebrates
Cell Tropism N.A.
Associated Disease Asymptomatic
Mode of Transmission Respiratory, oral droplets
VIPR DB link N.A.
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/ssdna-viruses-2011/w/ssdna_viruses/151/parvoviridae
Virus Host DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Parvoviridae

Publication Information

Paper Title Genome wide RNAi screening identifies host restriction factors critical for in vivo AAV transduction
Author's Name Miguel Manoa, Rudy Ippodrinoa , Lorena Zentilina , Serena Zacchigna, Mauro Giacca
Journal Name PNAS
Pubmed ID 26305933
Abstract Viral vectors based on the adeno-associated virus (AAV) hold great promise for in vivo gene transfer several unknowns, however, still limit the vectors broader and more efficient application. Here, we report the results of a high-throughput, whole-genome siRNA screening aimed at identifying cellular factors regulating AAV transduction. We identified 1,483 genes affecting vector efficiency more than 4-fold and up to 50-fold, either negatively or positively. Most of these factors have not previously been associated to AAV infection. The most effective siRNAs were independent from the virus serotype or analyzed cell type and were equally evident for single-stranded and self-complementary AAV vectors. A common characteristic of the most effective siRNAs was the induction of cellular DNA damage and activation of a cell cycle checkpoint. This information can be exploited for the development of more efficient AAV-based gene delivery procedures. Administration of the most effective siRNAs identified by the screening to the liver significantly improved in vivo AAV transduction efficiency.
Used Model HeLa, U2OS and MRC5 cell lines
DOI 10.1073/pnas.1503607112