| Gene Name | CLDN1 |
| HF Protein Name | Claudin-1 |
| HF Function | Essential for viral entry |
| Uniprot ID | O95832 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 9076 |
| Host Factor (HF) Name in Paper | CLDN1 |
| Gene synonyms | CLD1 SEMP1 |
| Ensemble Gene ID | ENSG00000163347 |
| Ensemble Transcript | ENST00000295522 |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0001618, GO:0005198, GO:0005737, GO:0005887, GO:0005923, GO:0007568, GO:0008065, GO:0016021, GO:0016323, GO:0016324, GO:0016328, GO:0016338, GO:0032496, GO:0042538, GO:0042802, GO:0045216, GO:0045471, GO:0051260, GO:0051291, GO:0061436, GO:0061772, GO:0070673, GO:0070830, GO:0071284, GO:0071346, GO:0071356, GO:0071548, GO:0071560, GO:0090557, GO:0097421, GO:1903348, GO:1903545, |
| MINT ID | O95832 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 603718 |
| PANTHER ID | PTHR12002;PTHR12002:SF92 |
| PDB ID(s) | N.A., |
| pfam ID | PF00822, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Dengue virus 2 |
| Virus Short Name | DENV 2 |
| Order | Unassigned |
| Virus Family | Flaviviridae |
| Virus Subfamily | N.A. |
| Genus | Flavivirus |
| Species | Dengue virus |
| Host | Human, mammals, mosquitoes and ticks |
| Cell Tropism | Phagocytes, hepatocytes |
| Associated Disease | Dengue fever |
| Mode of Transmission | Arthropod bite, mainly mosquitoes |
| VIPR DB link | http://www.viprbrc.org/brc/home.spg?decorator=flavi |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/flaviviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Flaviviridae |
| Paper Title | The interaction between claudin-1 and dengue viral prM/M protein for its entry |
| Author's Name | Pulin Che, Hengli Tang, Qianjun Li |
| Journal Name | Virology |
| Pubmed ID | 24074594 |
| Abstract | Dengue disease is becoming a huge public health concern around the world as more than one-third of the worlds population living in areas at risk of infection. In an effort to assess host factors interacting with dengue virus, we identified claudin-1, a major tight junction component, as an essential cell surface protein for dengue virus entry. When claudin-1 was knocked down in Huh 7.5 cells via shRNA, the amount of dengue virus entering host cells was reduced. Consequently, the progeny virus productions were decreased and denguevirus-induced CPE was prevented. Furthermore, restoring the expression of claudin-1 in the knockdown cells facilitated dengue virus entry. The interaction between claudin-1 and dengue viral prM protein was further demonstrated using the pull-down assay. Deletion of the extracellular loop 1 (ECL1) of claudin-1 abolished such interaction, so did point mutations C54A, C64A and I32M on ECL1. These results suggest that the interaction between viral protein prM and host protein claudin-1 was essential for dengue entry. Since host and viral factors involved in virus entry are promising therapeutic targets, determining the essential role of claudin-1 could lead to the discovery of entry inhibitors with attractive therapeutic potential against dengue disease. |
| Used Model | Huh 7.5 cells |
| DOI | 10.1016/j.virol.2013.08.009 |
