| Gene Name | HSPA9 |
| HF Protein Name | Stress-70 protein, mitochondrial |
| HF Function | GRP75 is involved in processing of host miRNA, hsa-mir-126, that down regulates Dengue Virus replication |
| Uniprot ID | P38646 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 3313 |
| Host Factor (HF) Name in Paper | GRP75 |
| Gene synonyms | GRP75 HSPA9B mt-HSP70 |
| Ensemble Gene ID | ENSG00000113013 |
| Ensemble Transcript | ENST00000297185 |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0003723, GO:0005524, GO:0005730, GO:0005737, GO:0005739, GO:0005759, GO:0005925, GO:0006457, GO:0006611, GO:0016226, GO:0030218, GO:0031625, GO:0035722, GO:0042645, GO:0043066, GO:0043209, GO:0045646, GO:0045647, GO:0051082, GO:0070062, GO:1902037, |
| MINT ID | P38646 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 182170 |
| PANTHER ID | PTHR19375 |
| PDB ID(s) | 3N8E, 4KBO, |
| pfam ID | PF00012, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Dengue virus 2 |
| Virus Short Name | DENV 2 |
| Order | Unassigned |
| Virus Family | Flaviviridae |
| Virus Subfamily | N.A. |
| Genus | Flavivirus |
| Species | Dengue virus |
| Host | Human, mammals, mosquitoes and ticks |
| Cell Tropism | Phagocytes, hepatocytes |
| Associated Disease | Dengue fever |
| Mode of Transmission | Arthropod bite, mainly mosquitoes |
| VIPR DB link | http://www.viprbrc.org/brc/home.spg?decorator=flavi |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/flaviviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Flaviviridae |
| Paper Title | Role of human GRP75 in miRNA mediated regulation of dengue virus replication |
| Author's Name | Pavan Kumar Kakumani, Guruprasad R. Medigeshi, Inderjeet Kaur, Pawan Malhotra, Sunil K. Mukherjee and Raj K. Bhatnagar |
| Journal Name | GENE |
| Pubmed ID | 27039024 |
| Abstract | In recent times, RNAi has emerged as an important defence system that regulates replication of pathogens in host cells. Many RNAi related host factors especially the host miRNAs play important roles in all intrinsic cellular functions, including viral infection. We have been working on identification of mammalian host factors involved in Dengue virus infection. In the present study, we identified Glucose Regulated Protein 75kDa (GRP75), as a host factor that is associated with dicer complex, in particular with HADHA (trifunctional enzyme subunit alpha, mitochondrial), an auxiliary component of dicer complex. Knockdown of GRP75 by respective siRNAs in Huh-7 cells resulted in the accumulation of dengue viral genomic RNA suggesting a role of GRP75 in regulating dengue virus replication in human cell lines. To elucidate the mode of action of GRP75, we over expressed the protein in Huh-7 cells and analysed the host miRNAs processing. The results revealed that, GRP75 is involved in processing of host miRNA, hsa-mir-126, that down regulates dengue virus replication. These findings suggest a regulatory role of human miRNA pathway especially GRP75 protein and hsa-mir-126 in dengue virus replication. These results thus provide insights into the role of miRNAs and RNAi machinery in dengue life cycle. |
| Used Model | Huh-7 and HEK293T cells |
| DOI | 10.1016/j.gene.2016.03.053 |
