| Gene Name | ISG15 |
| HF Protein Name | Ubiquitin-like protein ISG15 |
| HF Function | Restricts Dengue Virus replication |
| Uniprot ID | P05161 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 9636 |
| Host Factor (HF) Name in Paper | ISG15 |
| Gene synonyms | G1P2 UCRP |
| Ensemble Gene ID | ENSG00000187608 |
| Ensemble Transcript | ENST00000379389 |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0005576, GO:0005654, GO:0005829, GO:0016032, GO:0019941, GO:0019985, GO:0030501, GO:0031386, GO:0031397, GO:0032020, GO:0032480, GO:0032649, GO:0034340, GO:0042742, GO:0045071, GO:0045648, GO:0051607, GO:0060337, |
| MINT ID | P05161 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 147571 |
| PANTHER ID | N.A. |
| PDB ID(s) | 1Z2M, 2HJ8, 3PHX, 3PSE, 3R66, 3RT3, 3SDL, 5TL6, 5W8T, 5W8U, 6FFA, |
| pfam ID | PF00240, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Dengue virus 2 |
| Virus Short Name | DENV 2 |
| Order | Unassigned |
| Virus Family | Flaviviridae |
| Virus Subfamily | N.A. |
| Genus | Flavivirus |
| Species | Dengue virus |
| Host | Human, mammals, mosquitoes and ticks |
| Cell Tropism | Phagocytes, hepatocytes |
| Associated Disease | Dengue fever |
| Mode of Transmission | Arthropod bite, mainly mosquitoes |
| VIPR DB link | http://www.viprbrc.org/brc/home.spg?decorator=flavi |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/flaviviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Flaviviridae |
| Paper Title | Interferon-mediated ISG15 conjugation restricts dengue virus 2 replication |
| Author's Name | Takayuki Hishiki, QiEn Han, Kei-ichiro Arimoto, Kunitada Shimotohno, Tatsuhiko Igarashi, Subhash G. Vasudevan, Youichi Suzuki, Naoki Yamamoto |
| Journal Name | Biochemical and Biophysical Research Communications |
| Pubmed ID | 24769207 |
| Abstract | ISGylation, an ubiquitin-like post-translational modification by ISG15, has been reported to participate in the interferon (IFN)-mediated antiviral response. In this study, we analyzed the functional role of ISGylation in dengue virus 2 (DENV-2) replication. Overexpression of ISG15 was found to significantly suppress the amount of extracellular infectious virus released, while intracellular viral RNA was unaffected. This effect was not observed with a conjugation-defective ISG15 mutant. In addition, extracellular virus infectivity was decreased by ISG15 overexpression. To further clarify the role of ISGylation in the anti-DENV-2 response, we depleted endogenous ISG15 by RNA interference and analyzed the virus production in the absence or presence of type-I IFN. Results showed a significant reduction in extracellular DENV-2 RNA levels for cells treated with IFN, and that these DENV-2 RNA levels could be partially restored by the ISG15 knockdown. Among various DENV-2 proteins, NS3 and NS5 were subjected to the ISGylation. These results demonstrate that IFN-inducible ISGylation suppresses DENV-2 particle release, and that ISG15 is one of the mediators of IFN-induced inhibition of DENV-2 replication. ISG15 therefore functions as a host antiviral factor against DENV-2 infection. |
| Used Model | HeLa and HEK293T cells |
| DOI | 10.1016/j.bbrc.2014.04.081 |
