| Gene Name | NUP214 |
| HF Protein Name | Nuclear pore complex protein Nup214 |
| HF Function | Required for EV71 virus replication |
| Uniprot ID | P35658 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 8021 |
| Host Factor (HF) Name in Paper | NUP214 |
| Gene synonyms | CAIN CAN KIAA0023 |
| Ensemble Gene ID | ENSG00000126883 |
| Ensemble Transcript | ENST00000359428 [P35658-1];ENST00000411637 [P35658-4] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0005049, GO:0005634, GO:0005654, GO:0005829, GO:0005925, GO:0006110, GO:0006405, GO:0006406, GO:0006409, GO:0006606, GO:0006611, GO:0007077, GO:0008139, GO:0016032, GO:0016925, GO:0017056, GO:0019083, GO:0043231, GO:0043488, GO:0043657, GO:0044613, GO:0044615, GO:0046822, GO:0051726, GO:0060964, GO:0075733, GO:1900034, GO:1990876, |
| MINT ID | P35658 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 114350 |
| PANTHER ID | PTHR23193 |
| PDB ID(s) | 2OIT, 3FHC, 3FMO, 3FMP, 5DIS, |
| pfam ID | N.A., |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Enterovirus 71 |
| Virus Short Name | EV-71 |
| Order | Picornavirales |
| Virus Family | Picornaviridae |
| Virus Subfamily | N.A. |
| Genus | Enterovirus |
| Species | Enterovirus A |
| Host | Human, mammals |
| Cell Tropism | The gastrointestinal trac |
| Associated Disease | Hand, foot and mouth disease, meningitis |
| Mode of Transmission | Either fecal-oral or respiratory |
| VIPR DB link | https://www.viprbrc.org/brc/home.spg?decorator=picorna |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/234/picornaviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Picornaviridae |
| Paper Title | Validation-based insertional mutagenesis for identification of Nup214 as a host factor for EV71 replication in RD cells |
| Author's Name | Bei Wang, XiaoYu Zhang, Zhendong Zhao |
| Journal Name | Biochemical and Biophysical Research Communications |
| Pubmed ID | 23831628 |
| Abstract | Lentiviral validation-based insertional mutagenesis (VBIM) is a sophisticated, forward genetic approach that is used for the investigation of signal transduction in mammalian cells. Using VBIM, we conducted function-based genetic screening for host genes that affect enterovirus 71 (EV71) viral replication. This included host factors that are required for the life cycle of EV71 and host restriction factors that inhibit EV71 replication. Several cell clones, resistant to EV71, were produced using EV71 infection as a selection pressure and the nuclear pore protein 214 (Nup214) was identified as a host factor required for EV71 replication. In SD2-2, the corresponding VBIM lentivirus transformed clone, the expression of endogenous Nup214 was significantly down-regulated by the reverse inserted VBIM promoter. After Cre recombinase-mediated excision of the VBIM promoter, the expression of Nup214 recovered and the clone regained sensitivity to the EV71 infection. Furthermore, over-expression of Nup214 in the cells suggested that Nup214 was promoting EV71replication. Results of this study indicate that a successful mutagenesis strategy has been established for screening host genes related to viral replication. |
| Used Model | RD cells |
| DOI | 10.1016/j.bbrc.2013.06.101 |
