| Gene Name | IFIH1 |
| HF Protein Name | Interferon-induced helicase C domain-containing protein 1 |
| HF Function | Activates type-I IFN expression |
| Uniprot ID | Q9BYX4 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 64135 |
| Host Factor (HF) Name in Paper | MDA5 |
| Gene synonyms | MDA5 RH116 |
| Ensemble Gene ID | ENSG00000115267 |
| Ensemble Transcript | ENST00000263642 [Q9BYX4-1];ENST00000421365 [Q9BYX4-2] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0003677, GO:0003725, GO:0003727, GO:0004386, GO:0005524, GO:0005634, GO:0005829, GO:0008270, GO:0009597, GO:0009615, GO:0016032, GO:0016579, GO:0016925, GO:0032480, GO:0032727, GO:0032728, GO:0034344, GO:0035549, GO:0039528, GO:0039530, GO:0042802, GO:0043021, GO:0045087, GO:0051607, GO:0060760, GO:0071360, GO:1902741, GO:1904469, GO:2000778, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 182250 |
| PANTHER ID | N.A. |
| PDB ID(s) | 2RQB, 3B6E, 3GA3, 4GL2, |
| pfam ID | PF16739, PF00271, PF04851, PF11648, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Enterovirus 71 |
| Virus Short Name | EV-71 |
| Order | Picornavirales |
| Virus Family | Picornaviridae |
| Virus Subfamily | N.A. |
| Genus | Enterovirus |
| Species | Enterovirus A |
| Host | Human, mammals |
| Cell Tropism | The gastrointestinal trac |
| Associated Disease | Hand, foot and mouth disease, meningitis |
| Mode of Transmission | Either fecal-oral or respiratory |
| VIPR DB link | https://www.viprbrc.org/brc/home.spg?decorator=picorna |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/234/picornaviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Picornaviridae |
| Paper Title | MDA5 Plays a Crucial Role in Enterovirus 71 RNA- Mediated IRF3 Activation |
| Author's Name | Rei-Lin Kuo, Li-Ting Kao, Sue-Jane Lin, Robert Yung-Liang Wang, Shin-Ru Shih |
| Journal Name | PLOS ONE |
| Pubmed ID | 23650567 |
| Abstract | Induction of type-I interferons (IFNs), IFN-alpha/beta, is crucial to innate immunity against RNA virus infection. Cytoplasmic retinoic acid-inducible gene I (RIG-I)-like receptors, including RIG-I and melanoma differentiation-associated gene 5 (MDA5), are critical pathogen sensors for activation of type-I IFN expression in response to RNA virus infection. MDA5 is required for type-I IFN expression in mouse models in response to infection by picornaviruses, such as encephalomyocarditis virus (EMCV) and coxsackievirus B3. Enterovirus 71(EV71) belongs to picornaviridae and contains positive-stranded RNA genome that is linked with VPg protein at the 5 end. Although a recent study showed that EV71 3C protease could suppress RIG-I-mediated IFN-beta response, the cytoplasmic RIG-I-like receptor that is directly involved in the recognition of EV71 RNA remains unclear. Using EV71-derived RNA as an agonist, we demonstrate that MDA5is involved in EV71 RNA-mediated IRF3 activation and IFN-beta transcription. Our data also show that overexpression of the MDA5 protein reverses the suppression of IRF3 activation caused by EV71 infection. These results indicate that MDA5 is an important factor for EV71 RNA-activated type-I IFN expression. Furthermore, we also show that EV71 infection enhances MDA5 degradation and that the degradation could be inhibited by a broad spectrum caspase inhibitor. |
| Used Model | HeLa, Vero and RD cells |
| DOI | 10.1371/journal.pone.0063431 |
