Download Help

Virus Details


VHFID1965

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name TRIM24
HF Protein Name Transcription intermediary factor 1-alpha
HF Function Required for EV71 infection
Uniprot ID O15164
Protein Sequence View Fasta Sequence
NCBI Gene ID 8805
Host Factor (HF) Name in Paper TRIM24
Gene synonyms RNF82 TIF1 TIF1A
Ensemble Gene ID ENSG00000122779
Ensemble Transcript ENST00000343526 [O15164-1];ENST00000415680 [O15164-2]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0002039, GO:0003682, GO:0003713, GO:0004713, GO:0004842, GO:0005102, GO:0005634, GO:0005654, GO:0005719, GO:0005726, GO:0005829, GO:0006366, GO:0008270, GO:0008285, GO:0016567, GO:0016922, GO:0030163, GO:0031647, GO:0034056, GO:0042981, GO:0045892, GO:0045893, GO:0046777, GO:0055074, GO:0061630, GO:0070562, GO:0070577, GO:0071391, GO:1901796,
MINT ID O15164
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 603406
PANTHER ID N.A.
PDB ID(s) 2YYN, 3O33, 3O34, 3O35, 3O36, 3O37, 4YAB, 4YAD, 4YAT, 4YAX, 4YBM, 4YBS, 4YBT, 4YC9, 4ZQL, 5H1T, 5H1U, 5H1V,
pfam ID PF00439, PF00628, PF00643,
Drug Bank ID N.A.,
ChEMBL ID CHEMBL3108638
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Enterovirus 71
Virus Short Name EV-71
Order Picornavirales
Virus Family Picornaviridae
Virus Subfamily N.A.
Genus Enterovirus
Species Enterovirus A
Host Human, mammals
Cell Tropism The gastrointestinal trac
Associated Disease Hand, foot and mouth disease, meningitis
Mode of Transmission Either fecal-oral or respiratory
VIPR DB link https://www.viprbrc.org/brc/home.spg?decorator=picorna
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/234/picornaviridae
Virus Host DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Picornaviridae

Publication Information

Paper Title Human genome-wide RNAi screen reveals host factors required for enterovirus 71 replication
Author's Name Kan Xing Wu, Patchara Phuektes, Pankaj Kumar, Germaine Yen Lin Goh, Dimitri Moreau, Vincent Tak Kwong Chow, Frederic Bard & Justin Jang Hann Chu
Journal Name Nature Communications
Pubmed ID 27748395
Abstract Enterovirus 71 (EV71) is a neurotropic enterovirus without antivirals or vaccine, and its host-pathogen interactions remain poorly understood. Here we use a human genome-wide RNAi screen to identify 256 host factors involved in EV71 replication in human rhabdomyosarcoma cells. Enrichment analyses reveal overrepresentation in processes like mitotic cell cycle and transcriptional regulation. We have carried out orthogonal experiments to characterize the roles of selected factors involved in cell cycle regulation and endoplasmatic reticulum-associated degradation. We demonstrate nuclear egress of CDK6 in EV71 infected cells, and identify CDK6 and AURKB as resistance factors. NGLY1, which co-localizes with EV71 replication complexes at the endoplasmatic reticulum, supports EV71 replication. We confirm importance of these factors for EV71 replication in a human neuronal cell line and for coxsackievirus A16 infection. A small molecule inhibitor of NGLY1 reduces EV71 replication. This study provides a comprehensive map of EV71 host factors and reveals potential antiviral targets.
Used Model RD cells
DOI 10.1038/ncomms13150