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Virus Details


VHFID2972

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name DNM2
HF Protein Name Dynamin-2
HF Function Required for virus entry
Uniprot ID P50570
Protein Sequence View Fasta Sequence
NCBI Gene ID 1785
Host Factor (HF) Name in Paper DNM2
Gene synonyms DYN2
Ensemble Gene ID ENSG00000079805
Ensemble Transcript ENST00000355667 [P50570-1];ENST00000359692 [P50570-2];ENST00000389253 [P50570-4];ENST00000408974 [P50570-3];ENST00000585892 [P50570-5]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000086, GO:0000139, GO:0000266, GO:0001891, GO:0001917, GO:0002031, GO:0003281, GO:0003374, GO:0003924, GO:0005525, GO:0005634, GO:0005768, GO:0005794, GO:0005802, GO:0005813, GO:0005829, GO:0005874, GO:0005886, GO:0005905, GO:0005925, GO:0006355, GO:0006893, GO:0006897, GO:0006898, GO:0006909, GO:0007165, GO:0007283, GO:0008017, GO:0009416, GO:0010592, GO:0014069, GO:0017124, GO:0019886, GO:0019899, GO:0019901, GO:0030027, GO:0030426, GO:0030496, GO:0030512, GO:0030516, GO:0030666, GO:0030670, GO:0031623, GO:0031749, GO:0031966, GO:0032587, GO:0033572, GO:0035020, GO:0035904, GO:0036312, GO:0042220, GO:0043065, GO:0044351, GO:0045211, GO:0045334, GO:0045429, GO:0045893, GO:0048471, GO:0048489, GO:0048812, GO:0050699, GO:0050766, GO:0050998, GO:0050999, GO:0060976, GO:0061024, GO:0061025, GO:0070062, GO:0071245, GO:0071481, GO:0071732, GO:1900026, GO:1902856, GO:1903351, GO:1903358, GO:1903408, GO:1903526,
MINT ID P50570
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 160150
PANTHER ID PTHR11566;PTHR11566:SF23
PDB ID(s) 2YS1,
pfam ID PF01031, PF00350, PF02212, PF00169,
Drug Bank ID N.A.,
ChEMBL ID CHEMBL5812
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Echovirus 1
Virus Short Name E-1
Order Picornavirales
Virus Family Picornaviridae
Virus Subfamily N.A.
Genus Enterovirus
Species Enterovirus B
Host Human, mammals
Cell Tropism The gastrointestinal trac
Associated Disease Common cold
Mode of Transmission Either fecal-oral or respiratory
VIPR DB link https://www.viprbrc.org/brc/home.spg?decorator=picorna
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/234/picornaviridae
Virus Host DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Picornaviridae

Publication Information

Paper Title Echovirus 1 Endocytosis into Caveosomes Requires Lipid Rafts, Dynamin II, and Signaling Events
Author's Name Vilja Pietiainen, Varpu Marjomaki, Paula Upla, Lucas Pelkmans, Ari Helenius, and Timo Hyypia
Journal Name Molecular Biology of the Cell
Pubmed ID 15356270
Abstract Binding of echovirus 1 (EV1, a nonenveloped RNA virus) to the alpha2beta1 integrin on the cell surface is followed by endocytic internalization of the virus together with the receptor. Here, video-enhanced live microscopy revealed the rapid uptake of fluorescently labeled EV1 into mobile, intracellular structures, positive for green fluorescent protein-tagged caveolin-1. Partial colocalization of EV1 with SV40 (SV40) and cholera toxin, known to traffic via caveosomes, demonstrated that the vesicles were caveosomes. The initiation of EV1 infection was dependent on dynamin II, cholesterol, and protein phosphorylation events. Brefeldin A, a drug that prevents SV40 transport, blocked the EV1 infection cycle, whereas drugs that disrupt the cellular cytoskeleton had no effect. In situ hybridization revealed the localization of viral RNA with endocytosed viral capsid proteins in caveosomes before initiation of viral replication. Thus, both the internalization of EV1 to caveosomes and subsequent events differ clearly from caveolar endocytosis of SV40 because EV1 uptake is fast and independent of actin and EV1 is not sorted further to sER from caveosomes. These results shed further light on the cell entry of nonenveloped viral pathogens and illustrate the use of viruses as probes to dissect caveolin-associated endocytic pathways.
Used Model CV-1 and SAOS-alpha2beta1 cells
DOI 10.1091/mbc.E04-01-0070