| Gene Name | PLCB3 |
| HF Protein Name | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 |
| HF Function | Required for PV virus infection |
| Uniprot ID | Q01970 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 5331 |
| Host Factor (HF) Name in Paper | PLCB3 |
| Gene synonyms | N.A. |
| Ensemble Gene ID | ENSG00000149782 |
| Ensemble Transcript | ENST00000279230 [Q01970-1];ENST00000325234 [Q01970-2];ENST00000540288 [Q01970-1] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0003073, GO:0004435, GO:0004629, GO:0004871, GO:0005509, GO:0005516, GO:0005654, GO:0005829, GO:0007186, GO:0007223, GO:0016020, GO:0016042, GO:0032991, GO:0035556, GO:0043647, GO:0045296, |
| MINT ID | Q01970 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 600230 |
| PANTHER ID | PTHR10336 |
| PDB ID(s) | 3OHM, 4GNK, 4QJ3, 4QJ4, 4QJ5, |
| pfam ID | PF00168, PF09279, PF00388, PF00387, PF08703, |
| Drug Bank ID | N.A., |
| ChEMBL ID | CHEMBL5449 |
| Organism | Homo sapiens (Human) |
| Virus Name | Polio Virus |
| Virus Short Name | PV |
| Order | Picornavirales |
| Virus Family | Picornaviridae |
| Virus Subfamily | N.A. |
| Genus | Enterovirus |
| Species | Enterovirus C |
| Host | Human, mammals |
| Cell Tropism | The gastrointestinal tract |
| Associated Disease | Poliomyelitis |
| Mode of Transmission | Either fecal-oral or respiratory |
| VIPR DB link | https://www.viprbrc.org/brc/home.spg?decorator=picorna |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/234/picornaviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Picornaviridae |
| Paper Title | Comparative RNAi screening reveals host factors involved in enterovirus infection of polarized endothelial monolayers |
| Author's Name | Carolyn B. Coyne, Rebecca Bozym, Stefanie A. Morosky, Sheri L. Hanna, Amitava Mukherjee, Matthew Tudor, Kwang Sik Kim, and Sara Cherry |
| Journal Name | Cell Host & Microbe |
| Pubmed ID | 21238948 |
| Abstract | Enteroviruses, including coxsackievirus B (CVB) and poliovirus (PV), can access the CNS through the blood brain barrier (BBB) endothelium to cause aseptic meningitis. To identify cellular components required for CVB and PV infection of human brain microvascular endothelial cells, an in vitro BBB model, we performed comparative RNAi screens and identified 117 genes that influenced infection. Whereas a large proportion of genes whose depletion enhanced infection (17 of 22) were broadly antienteroviral, only 46 of the 95 genes whose depletion inhibited infection were required by both CVB and PV and included components of cell signaling pathways such as adenylate cyclases. Downregulation of genes including Rab GTPases, Src tyrosine kinases, and tyrosine phosphatases displayed specificity in their requirement for either CVB or PV infection. These findings highlight the pathways hijacked by enteroviruses for entry and replication in the BBB endothelium, a specialized and clinically relevant cell type for these viruses. |
| Used Model | HBMEC and Caco-2 cells |
| DOI | 10.1016/j.chom.2011.01.001 |
