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Virus Details


VHFID3064

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name VCP
HF Protein Name Transitional endoplasmic reticulum ATPase
HF Function Required for Poliovirus replication
Uniprot ID P55072
Protein Sequence View Fasta Sequence
NCBI Gene ID 7415
Host Factor (HF) Name in Paper VCP
Gene synonyms N.A.
Ensemble Gene ID ENSG00000165280
Ensemble Transcript ENST00000358901
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000502, GO:0003723, GO:0005524, GO:0005576, GO:0005634, GO:0005654, GO:0005737, GO:0005783, GO:0005789, GO:0005811, GO:0005829, GO:0006281, GO:0006302, GO:0006457, GO:0006479, GO:0006734, GO:0006888, GO:0006914, GO:0006919, GO:0006974, GO:0008289, GO:0010498, GO:0010918, GO:0016236, GO:0016567, GO:0016579, GO:0016887, GO:0018279, GO:0019079, GO:0019903, GO:0019904, GO:0019985, GO:0030433, GO:0030968, GO:0030970, GO:0031334, GO:0031593, GO:0031625, GO:0032436, GO:0032510, GO:0032991, GO:0034098, GO:0034214, GO:0034774, GO:0035578, GO:0035800, GO:0035861, GO:0036435, GO:0036503, GO:0036513, GO:0042288, GO:0042802, GO:0042981, GO:0043161, GO:0043209, GO:0043231, GO:0043312, GO:0043531, GO:0044389, GO:0045184, GO:0045732, GO:0046034, GO:0048471, GO:0051260, GO:0055085, GO:0061857, GO:0070062, GO:0070842, GO:0070987, GO:0071712, GO:0072389, GO:0090263, GO:0097352, GO:1903006, GO:1903007, GO:1903715, GO:1903862, GO:1904288, GO:1904813, GO:1904949, GO:1990381, GO:1990730, GO:2001171,
MINT ID P55072
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 167320
PANTHER ID N.A.
PDB ID(s) 3EBB, 3HU1, 3HU2, 3HU3, 3QC8, 3QQ7, 3QQ8, 3QWZ, 3TIW, 4KDI, 4KDL, 4KLN, 4KO8, 4KOD, 4P0A, 5B6C, 5C18, 5C19, 5C1A, 5C1B, 5DYG, 5DYI, 5EPP, 5FTJ, 5FTK, 5FTL, 5FTM, 5FTN, 5GLF, 5IFS, 5IFW, 5KIW, 5KIY, 5X4L,
pfam ID PF00004, PF02933, PF02359, PF09336,
Drug Bank ID DB04395,
ChEMBL ID CHEMBL1075145
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Polio Virus
Virus Short Name PV
Order Picornavirales
Virus Family Picornaviridae
Virus Subfamily N.A.
Genus Enterovirus
Species Enterovirus C
Host Human, mammals
Cell Tropism The gastrointestinal trac
Associated Disease Poliomyelitis
Mode of Transmission Either fecal-oral or respiratory
VIPR DB link https://www.viprbrc.org/brc/home.spg?decorator=picorna
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/234/picornaviridae
Virus Host DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Picornaviridae

Publication Information

Paper Title Valosin-Containing Protein (VCP/p97) Is Required for Poliovirus Replication and Is Involved in Cellular Protein Secretion Pathway in Poliovirus Infection
Author's Name Minetaro Arita, Takaji Wakita, and Hiroyuki Shimizu
Journal Name Journal Of Virology
Pubmed ID 22379090
Abstract Poliovirus (PV) modifies membrane-trafficking machinery in host cells for its viral RNA replication. To date, ARF1, ACBD3, BIG1/BIG2, GBF1, RTN3, and PI4KB have been identified as host factors of enterovirus (EV), including PV, involved in membrane traffic. In this study, we performed small interfering RNA (siRNA) screening targeting membrane-trafficking genes for host factors required for PV replication. We identified valosin-containing protein (VCP/p97) as a host factor of PV replication required after viral protein synthesis, and its ATPase activity was essential for PV replication. VCP colocalized with viral proteins 2BC/2C and 3AB/3B in PV-infected cells and showed an interaction with 2BC and 3AB but not with 2C and 3A. Knockdown of VCP did not suppress the replication of coxsackievirus B3 or Aichi virus. A VCP-knockdown-resistant PV mutant had an A4881G (a mutation of E253G in 2C) mutation, which is known as a determinant of a secretion inhibition-negative phenotype. However, knockdown of VCP did not affect the inhibition of cellular proteinsecretion caused by overexpression of each individual viral protein. These results suggested that VCP is a host factor required for viral RNA replication of PV among membrane-trafficking proteins and provides a novel link between cellular protein secretion and viral RNA replication.
Used Model RD and HEK293 cells
DOI 10.1128/JVI.00114-12