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Virus Details


VHFID3192

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name NFKB2
HF Protein Name Nuclear factor NF-kappa-B p100 subunit
HF Function Interacts with regulatory proteins of virus
Uniprot ID Q00653
Protein Sequence View Fasta Sequence
NCBI Gene ID 4791
Host Factor (HF) Name in Paper p100
Gene synonyms LYT10
Ensemble Gene ID ENSG00000077150
Ensemble Transcript ENST00000189444 [Q00653-4];ENST00000369966 [Q00653-1];ENST00000428099 [Q00653-4]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000122, GO:0000978, GO:0000981, GO:0001077, GO:0002268, GO:0002467, GO:0003682, GO:0003700, GO:0003713, GO:0005634, GO:0005654, GO:0005737, GO:0005829, GO:0006355, GO:0006954, GO:0007249, GO:0007568, GO:0030198, GO:0032481, GO:0032496, GO:0033257, GO:0034097, GO:0038061, GO:0045087, GO:0045944, GO:0048511, GO:0048536, GO:0051092,
MINT ID Q00653
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 164012
PANTHER ID PTHR24169
PDB ID(s) 1A3Q, 2D96, 3DO7, 4OT9,
pfam ID PF12796, PF00531, PF16179, PF00554,
Drug Bank ID DB00945, DB05487, DB01296, DB05212, DB05767, DB05464, DB05451, DB05471,
ChEMBL ID CHEMBL3003
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Equine Arteritis Virus
Virus Short Name EAV
Order Nidovirales
Virus Family Arteriviridae
Virus Subfamily N.A.
Genus Equartevirus
Species Equine Arteritis Virus
Host Vertebrates
Cell Tropism Primarily lung macrophages and later in lymph nodes
Associated Disease Vascular lesions, fever, edema, abortion
Mode of Transmission Genital and respiratory tract secretions.
VIPR DB link N.A.
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/220/arteriviridae
Virus Host DB link N.A.

Publication Information

Paper Title Equine arteritis virus non-structural protein 1, an essential factor for viral subgenomic mRNA synthesis, interacts with the cellular transcription co-factor p100
Author's Name Marieke A. Tijms and Eric J. Snijder
Journal Name Journal of General Virology
Pubmed ID 12917451
Abstract Non-structural protein 1 (nsp1), the N-terminal subunit of the replicase polyprotein of the arterivirus Equine arteritis virus (EAV), is essential for viral subgenomic mRNA synthesis, but fully dispensable for genome replication. However, at the molecular level, the role of nsp1 in EAV subgenomic mRNA synthesis is poorly understood. A yeast two-hybrid screen did not reveal interactions between EAV nsp1 and other viral non-structural proteins or the nucleocapsid protein, although both nsp1 and the nucleocapsid protein were found to form homomers. Subsequently, a yeast two-hybrid screen of a HeLa cell cDNA library was performed using nsp1 as bait. Remarkably, this analysis revealed (potential) interactions between EAV nsp1 and factors that are involved in host cell transcriptional regulation. The interaction of nsp1 with one of these proteins, p100, a transcription co-activator that also interacts with regulatory proteins of other viruses, was confirmed by mutual co-immunoprecipitation from lysates of EAV-susceptible mammalian cells.
Used Model HeLa cell
DOI 10.1099/vir.0.19297-0