| Gene Name | EIF4G1 |
| HF Protein Name | Eukaryotic translation initiation factor 4 gamma 1 |
| HF Function | Essential for internal initiation of translation |
| Uniprot ID | Q04637 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 1981 |
| Host Factor (HF) Name in Paper | EIF4G1 |
| Gene synonyms | EIF4F EIF4G EIF4GI |
| Ensemble Gene ID | ENSG00000114867 |
| Ensemble Transcript | ENST00000342981 [Q04637-8];ENST00000346169 [Q04637-1];ENST00000350481 [Q04637-5];ENST00000352767 [Q04637-9];ENST00000382330 [Q04637-9];ENST00000392537 [Q04637-4];ENST00000414031 [Q04637-3];ENST00000424196 [Q04637-9];ENST00000434061 [Q04637-7];ENST00000435046 [Q04637-6] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000184, GO:0000289, GO:0001662, GO:0002191, GO:0003723, GO:0003729, GO:0003743, GO:0005524, GO:0005634, GO:0005737, GO:0005829, GO:0005844, GO:0006412, GO:0006413, GO:0006446, GO:0007005, GO:0008135, GO:0008190, GO:0008284, GO:0010507, GO:0010801, GO:0010942, GO:0016020, GO:0016032, GO:0016281, GO:0030307, GO:0031369, GO:0031669, GO:0032270, GO:0032502, GO:0032947, GO:0033138, GO:0034645, GO:0042802, GO:0043488, GO:0045296, GO:0045666, GO:0060964, GO:0080135, GO:0097009, GO:1900087, GO:1901215, GO:1905537, GO:1905606, GO:1905612, GO:1905618, GO:1905696, |
| MINT ID | Q04637 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 600495 |
| PANTHER ID | PTHR23253:SF10 |
| PDB ID(s) | 1LJ2, 1UG3, 2W97, 4AZA, 4F02, 5EHC, 5EI3, 5EIR, 5T46, |
| pfam ID | PF02847, PF02854, PF02020, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Hepatitis A virus |
| Virus Short Name | HAV |
| Order | Picornavirales |
| Virus Family | Picornaviridae |
| Virus Subfamily | N.A. |
| Genus | Hepatovirus |
| Species | Hepatitis A virus |
| Host | Human, vertebrates |
| Cell Tropism | Liver |
| Associated Disease | Mild hepatitis |
| Mode of Transmission | Fecal-oral and blood |
| VIPR DB link | https://www.viprbrc.org/brc/home.spg?decorator=picorna |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/234/picornaviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Picornaviridae |
| Paper Title | Intact Eukaryotic Initiation Factor 4G Is Required for Hepatitis A Virus Internal Initiation of Translation |
| Author's Name | Borman AM, Kean KM. |
| Journal Name | Virology |
| Pubmed ID | 9344915 |
| Abstract | The requirements for optimal activity of the hepatitis A virus (HAV) internal ribosome entry segment (IRES) differ substantially from those of other picornavirus IRESes. One such difference is that, to date, the HAV IRES is the only one whose efficiency is severely inhibited in the presence of the picornaviral 2A proteinase. Here we describe experiments designed to dissect the mechanism of proteinase-mediated inhibition of HAV translation. Using dicistronic mRNAs translated in vitro, we show that the HAV IRES is inhibited by the foot-and-mouth disease virus Lb proteinase, as well as by the human rhinovirus 2A proteinase. Furthermore, using mutant Lb proteinase, we demonstrate that proteolytic activity is required for inhibition of HAV IRES activity. Translation inhibition correlated closely with the extent of cleavage of the one identified common cellular target for the 2A and Lb proteinases, eukaryotic initiation factor (eIF) 4G, a component of the eIF4F cap-binding protein complex. Total rescue of HAV IRES activity was possible if purified eIF4F was added to translation extracts. In contrast, if the added eIF4F contained cleaved eIF4G, no rescue of HAV IRES activity was evidenced. Thus the HAV IRES requires intact eIF4G for activity. This is unique among the picornavirus IRESes studied to date and may help explain why HAV does not inhibit host cell translation during viral infection. |
| Used Model | HeLa cells |
| DOI | 10.1006/viro.1997.8761 |
