| Gene Name | PCBP2 |
| HF Protein Name | Poly(rC)-binding protein 2 |
| HF Function | Essential for internal initiation of translation |
| Uniprot ID | Q15366 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 5094 |
| Host Factor (HF) Name in Paper | PCBP2 |
| Gene synonyms | N.A. |
| Ensemble Gene ID | ENSG00000197111 |
| Ensemble Transcript | ENST00000359282 [Q15366-4];ENST00000359462 [Q15366-2];ENST00000437231 [Q15366-7];ENST00000439930 [Q15366-1];ENST00000447282 [Q15366-5];ENST00000455667 [Q15366-7];ENST00000546463 [Q15366-3];ENST00000548933 [Q15366-5];ENST00000552296 [Q15366-6];ENST00000552819 [Q15366-8] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000398, GO:0003677, GO:0003723, GO:0005634, GO:0005654, GO:0005737, GO:0005829, GO:0005925, GO:0016020, GO:0016070, GO:0016071, GO:0019899, GO:0030529, GO:0031625, GO:0032480, GO:0039694, GO:0043161, GO:0045087, GO:0050687, GO:0051607, GO:0070062, GO:0075522, |
| MINT ID | Q15366 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 601210 |
| PANTHER ID | N.A. |
| PDB ID(s) | 2AXY, 2JZX, 2P2R, 2PQU, 2PY9, |
| pfam ID | PF00013, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Hepatitis A virus |
| Virus Short Name | HAV |
| Order | Picornavirales |
| Virus Family | Picornaviridae |
| Virus Subfamily | N.A. |
| Genus | Hepatovirus |
| Species | Hepatitis A virus |
| Host | Human, vertebrates |
| Cell Tropism | Liver |
| Associated Disease | Mild hepatitis |
| Mode of Transmission | Fecal-oral and blood |
| VIPR DB link | https://www.viprbrc.org/brc/home.spg?decorator=picorna |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/234/picornaviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Picornaviridae |
| Paper Title | Interaction of Poly(rC) Binding Protein 2 with the 59 Noncoding Region of Hepatitis A Virus RNA and Its Effects on Translation |
| Author's Name | Judith Graff, John Cha, Lawrence B. Blyn, and Ellie Ehrenfeld |
| Journal Name | Journal Of Virology |
| Pubmed ID | 9811700 |
| Abstract | Utilization of internal ribosome entry segment (IRES) structures in the 5 noncoding region (5NCR) of picornavirus RNAs for initiation of translation requires a number of host cell factors whose distribution may vary in different cells and whose requirement may vary for different picornaviruses. We have examined the requirement of the cellular protein poly(rC) binding protein 2 (PCBP2) for hepatitis A virus (HAV) RNA translation. PCBP2 has recently been identified as a factor required for translation and replication of poliovirus (PV) RNA. PCBP2 was shown to be present in FRhK-4 cells, which are permissive for growth of HAV, as it is in HeLa cells, which support translation of HAV RNA but which have not been reported to host replication of the virus. Competition RNA mobility shift assays showed that the 5NCR of HAV RNA competed for binding of PCBP2 with a probe representing stem-loop IV of the PV 5NCR. The binding site on HAV RNA was mapped to nucleotides 1 to 157, which includes a pyrimidine-rich sequence. HeLa cell extracts that had been depleted of PCBP2 by passage over a PV stem-loop IV RNA affinity column supported only low levels of HAV RNA translation. Translation activity was restored upon addition of recombinant PCBP2 to the depleted extract. Removal of the 5-terminal 138 nucleotides of the HAV RNA, or removal of the entire IRES, eliminated the dependence of HAV RNA translation on PCBP2. |
| Used Model | HeLa cells |
| DOI | N.A. |
