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Virus Details


VHFID3208

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name SIRT1
HF Protein Name NAD-dependent protein deacetylase sirtuin-1
HF Function SIRT1 enhances the activity of HBV core promoter by targeting transcription factor AP-1
Uniprot ID Q96EB6
Protein Sequence View Fasta Sequence
NCBI Gene ID 23411
Host Factor (HF) Name in Paper SIRT1
Gene synonyms SIR2L1
Ensemble Gene ID ENSG00000096717
Ensemble Transcript ENST00000212015 [Q96EB6-1]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000012, GO:0000122, GO:0000183, GO:0000720, GO:0000731, GO:0000790, GO:0001046, GO:0001077, GO:0001525, GO:0001542, GO:0001678, GO:0001934, GO:0001938, GO:0002039, GO:0002821, GO:0003714, GO:0004407, GO:0005634, GO:0005635, GO:0005637, GO:0005654, GO:0005677, GO:0005719, GO:0005720, GO:0005730, GO:0005737, GO:0005739, GO:0005829, GO:0006260, GO:0006281, GO:0006325, GO:0006342, GO:0006343, GO:0006344, GO:0006346, GO:0006364, GO:0006476, GO:0006642, GO:0006974, GO:0006979, GO:0007179, GO:0007283, GO:0007346, GO:0007517, GO:0007569, GO:0008022, GO:0008134, GO:0008284, GO:0009267, GO:0010629, GO:0010875, GO:0010883, GO:0010906, GO:0010934, GO:0014068, GO:0016032, GO:0016239, GO:0016567, GO:0016575, GO:0016605, GO:0017136, GO:0018394, GO:0019213, GO:0019899, GO:0030225, GO:0030308, GO:0030512, GO:0031393, GO:0031648, GO:0031937, GO:0032007, GO:0032071, GO:0032088, GO:0032868, GO:0032922, GO:0033158, GO:0033210, GO:0033553, GO:0033558, GO:0034391, GO:0034979, GO:0034983, GO:0035257, GO:0,
MINT ID Q96EB6
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 604479
PANTHER ID N.A.
PDB ID(s) 4I5I, 4IF6, 4IG9, 4KXQ, 4ZZH, 4ZZI, 4ZZJ, 5BTR,
pfam ID PF02146,
Drug Bank ID DB05073,
ChEMBL ID CHEMBL4506
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Hepatitis B virus
Virus Short Name HBV
Order Unassigned
Virus Family Hepadnaviridae
Virus Subfamily N.A.
Genus Orthohepadnavirus
Species Hepatitis B virus
Host Human, mammals
Cell Tropism Hepatocytes
Associated Disease Hepatitis, hepatocellular carcinoma(chronic infections), cirrhosis
Mode of Transmission Sexual contact, blood, maternal-neonatal
VIPR DB link N.A.
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/reverse-transcribing-dna-and-rna-viruses-2011/w/rt_viruses/155/hepadnaviridae
Virus Host DB link N.A.

Publication Information

Paper Title Sirtuin 1 Regulates Hepatitis B Virus Transcription and Replication by Targeting Transcription Factor AP-1
Author's Name Ji-Hua Ren, Ying Tao, Zhen-Zhen Zhang, Wei-Xian Chen, Xue-Fei Cai, Ke Chen, Ben C. B. Ko, Chun-Li Song, Long-Kuan Ran, Wan-Yu Li, Ai-Long Huang, Juan Chen
Journal Name Journal Of Virology
Pubmed ID 24335313
Abstract Chronic hepatitis B virus (HBV) infection is a major risk factor for liver cirrhosis and hepatocellular carcinoma. Nevertheless, the molecular mechanism of HBV replication remains elusive. SIRT1 is a class III histone deacetylase that is a structure component of the HBV cccDNA minichromosome. In this study, we found by using microarray-based gene expression profiling analysis that SIRT1 was upregulated in HBV-expressing cells. Gene silencing of SIRT1 significantly inhibited HBV DNA replicative intermediates, 3.5-kb mRNA, and core protein levels. In contrast, the overexpression of SIRT1 augmented HBV replication. Furthermore, SIRT1 enhanced the activity of HBV core promoter by targeting transcription factor AP-1. The c-Jun subunit of AP-1 was bound to the HBV core promoter region, as demonstrated by using a chromatin immunoprecipitation assay. Mutation of AP-1 binding site or knockdown of AP-1 abolished the effect of SIRT1 on HBV replication. Finally, SIRT1 inhibitor sirtinol also suppressed the HBV DNA replicative intermediate, as well as 3.5-kb mRNA. Our study identified a novel host factor, SIRT1, which may facilitate HBV replication in hepatocytes. These data suggest a rationale for the use of SIRT1 inhibitor in the treatment of HBV infection.
Used Model HepG2, Huh, HepG2.2.15 and HepG2-HBV1.1 cells
DOI 10.1128/JVI.02861-13