| Gene Name | PRPF31 |
| HF Protein Name | U4/U6 small nuclear ribonucleoprotein Prp31 |
| HF Function | Involves in the cccDNA production in HBV-replicating cells |
| Uniprot ID | Q8WWY3 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 26121 |
| Host Factor (HF) Name in Paper | PRPF31 |
| Gene synonyms | PRP31 |
| Ensemble Gene ID | ENSG00000105618 |
| Ensemble Transcript | ENST00000321030 [Q8WWY3-1];ENST00000419967 [Q8WWY3-4];ENST00000610903 [Q8WWY3-1];ENST00000612749 [Q8WWY3-4];ENST00000613693 [Q8WWY3-1];ENST00000614518 [Q8WWY3-4];ENST00000615175 [Q8WWY3-4];ENST00000616732 [Q8WWY3-1];ENST00000618595 [Q8WWY3-4];ENST00000618937 [Q8WWY3-1];ENST00000619220 [Q8WWY3-4];ENST00000619391 [Q8WWY3-4];ENST00000619439 [Q8WWY3-1];ENST00000619956 [Q8WWY3-1];ENST00000620142 [Q8WWY3-1];ENST00000620861 [Q8WWY3-1];ENST00000621588 [Q8WWY3-4];ENST00000622300 [Q8WWY3-1];ENST00000622387 [Q8WWY3-4];ENST00000622636 [Q8WWY3-4] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000244, GO:0000398, GO:0003723, GO:0005634, GO:0005654, GO:0005684, GO:0005687, GO:0005690, GO:0015030, GO:0016607, GO:0030621, GO:0030622, GO:0043021, GO:0046540, GO:0070990, GO:0071005, GO:0071011, GO:0071166, GO:0071339, |
| MINT ID | Q8WWY3 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 600138 |
| PANTHER ID | PTHR13904 |
| PDB ID(s) | 2OZB, 3JCR, 3SIU, 3SIV, 5O9Z, |
| pfam ID | PF01798, PF09785, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Hepatitis B virus |
| Virus Short Name | HBV |
| Order | Unassigned |
| Virus Family | Hepadnaviridae |
| Virus Subfamily | N.A. |
| Genus | Orthohepadnavirus |
| Species | Hepatitis B virus |
| Host | Human, mammals |
| Cell Tropism | Hepatocytes |
| Associated Disease | Hepatitis, hepatocellular carcinoma(chronic infections), cirrhosis |
| Mode of Transmission | Sexual contact, blood, maternal-neonatal |
| VIPR DB link | N.A. |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/reverse-transcribing-dna-and-rna-viruses-2011/w/rt_viruses/155/hepadnaviridae |
| Virus Host DB link | N.A. |
| Paper Title | Host factor PRPF31 is involved in cccDNA production in HBV-replicating cells |
| Author's Name | Wataru Kinoshitaa, Naoki Oguraa, Koichi Watashi, Takaji Wakita |
| Journal Name | Biochemical and Biophysical Research Communications |
| Pubmed ID | 27864147 |
| Abstract | Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) plays a central role in chronic HBV infection and replication, and is an important factor for HBV surface antigen loss indicating the endpoint of HBV treatment. However, there is a known problem that current anti-HBV drugs, including interferons and nucleos(t)ide analogues, reduce HBV replication but have a little or no effect on reducing cccDNA. Therefore, the development of new therapeutic agents is necessary to eradicate cccDNA. In this study, we identified pre-mRNA processing factor 31 (PRPF31) by siRNA screening as a factor associated with cccDNA. PRPF31 knockdown by siRNA decreased cccDNA formation without serious cytotoxicity. In rescue experiments, expression of siRNA-resistant PRPF31 recovered cccDNA formation. PRPF31 knockdown did not affect HBV core protein and HBV core DNA levels in HBV-replicating cells. Chromatin immunoprecipitation and immunoprecipitation assays revealed an association between PRPF31 and cccDNA. Furthermore, co-overexpression of PRPF31 and HBx enhanced cccDNA formation in HepAD38Â cells. Taken together, the present findings suggest that the interaction between PRPF31 and HBx may be a novel target for anti-HBV treatment. |
| Used Model | HepG2 cells and HepAD38 cells |
| DOI | 10.1016/j.bbrc.2016.11.085 |
