Download Help

Virus Details


VHFID3219

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name PARP1
HF Protein Name Poly [ADP-ribose] polymerase 1
HF Function Antiviral protein
Uniprot ID P09874
Protein Sequence View Fasta Sequence
NCBI Gene ID 142
Host Factor (HF) Name in Paper PARP1
Gene synonyms ADPRT PPOL
Ensemble Gene ID ENSG00000143799
Ensemble Transcript ENST00000366794
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000122, GO:0000715, GO:0000717, GO:0000723, GO:0000724, GO:0000784, GO:0000977, GO:0001228, GO:0003677, GO:0003723, GO:0003910, GO:0003950, GO:0005634, GO:0005635, GO:0005654, GO:0005667, GO:0005730, GO:0005739, GO:0006273, GO:0006281, GO:0006293, GO:0006294, GO:0006295, GO:0006296, GO:0006302, GO:0006366, GO:0006471, GO:0006915, GO:0006974, GO:0007005, GO:0007179, GO:0008134, GO:0008270, GO:0010332, GO:0010613, GO:0010990, GO:0016020, GO:0016540, GO:0018312, GO:0019899, GO:0019901, GO:0023019, GO:0030225, GO:0030331, GO:0032042, GO:0032869, GO:0032991, GO:0032993, GO:0033148, GO:0033683, GO:0034599, GO:0034644, GO:0036211, GO:0042769, GO:0042802, GO:0042826, GO:0043504, GO:0044030, GO:0045944, GO:0047485, GO:0050790, GO:0051103, GO:0051287, GO:0051901, GO:0060391, GO:0070212, GO:0070412, GO:0070911, GO:0071294, GO:1901216, GO:1903376, GO:1903518, GO:1903827, GO:1904044, GO:1904357, GO:1904646, GO:1904762, GO:1990404, GO:1990966, GO:2000679, GO:2001170,
MINT ID P09874
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 173870
PANTHER ID N.A.
PDB ID(s) 1UK0, 1UK1, 1WOK, 2COK, 2CR9, 2CS2, 2DMJ, 2JVN, 2L30, 2L31, 2N8A, 2RCW, 2RD6, 2RIQ, 3GJW, 3GN7, 3L3L, 3L3M, 3OD8, 3ODA, 3ODC, 3ODE, 4AV1, 4DQY, 4GV7, 4HHY, 4HHZ, 4L6S, 4OPX, 4OQA, 4OQB, 4PJT, 4R5W, 4R6E, 4RV6, 4UND, 4UXB, 4XHU, 4ZZZ, 5A00, 5DS3, 5HA9, 5KPN, 5KPO, 5KPP, 5KPQ, 5WRQ, 5WRY, 5WRZ, 5WS0, 5WS1, 5WTC, 6BHV,
pfam ID PF00533, PF08063, PF00644, PF02877, PF05406, PF00645,
Drug Bank ID DB04010, DB03509, DB03073, DB02498, DB02701, DB02690, DB09074, DB12332, DB07330, DB07232,
ChEMBL ID CHEMBL3105
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Hepatitis B virus
Virus Short Name HBV
Order Unassigned
Virus Family Hepadnaviridae
Virus Subfamily N.A.
Genus Orthohepadnavirus
Species Hepatitis B virus
Host Human, mammals
Cell Tropism Hepatocytes
Associated Disease Hepatitis, hepatocellular carcinoma(chronic infections), cirrhosis
Mode of Transmission Sexual contact, blood, maternal-neonatal
VIPR DB link N.A.
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/reverse-transcribing-dna-and-rna-viruses-2011/w/rt_viruses/155/hepadnaviridae
Virus Host DB link N.A.

Publication Information

Paper Title Reduced ADP-ribosylation by PARP1 natural polymorphism V762A and by PARP1 inhibitors enhance Hepatitis B virus replication
Author's Name H. L. Ko, H. J. Ng, E. H. Goh and E. C. Ren
Journal Name Journal Of Viral Hepatitis
Pubmed ID 23910651
Abstract HepG2 and Huh7 cell lines are frequently used as models to study viral hepatitis and hepatocellular carcinoma. However, they exhibit significantly different capacities in their ability to support hepatitis B virus (HBV) replication. To investigate the basis for this, transcription factor-binding motifs at the HBV core promoter (HBVCP) were tested in luciferase reporter constructs to identify the possible role of host factors. Among the transcription factors screened: PARP1, SP1, HNF4alpha, HNF3, hB1F and HNF1, deletion of the PARP1 binding motif abrogated transcriptional activity at the HBVCP in HepG2 but not Huh7 cells. Sequencing of the PARP1 gene revealed that HepG2 cells carried an Ala762 allele which has low ADP-ribosylation activity, which was shown to have increased PARP1 binding affinity to its cognate motif thus resulting in higher transcriptional activity. PARP1 inhibitors that are being developed as broad cancer therapeutics also target PARP1 ADP-ribosylation enzymatic function. Four PARP1 inhibitors: PJ-34, ABT888, AZD2281 and AG014699 were tested for their effect on HBV replication. All four small molecules effectively enhanced HBV replication in vitro, confirming the role of PARP1 in HBV replication and that alteration of ADP-ribosylation by mutation or drugs can affect HBV replication. Our data demonstrate that natural polymorphisms in the host affecting proteins such as PARP1 can have a significant effect on HBV replication. Hence, patients who are infected with HBV and are on clinical trials involving PARP1 inhibitors may be at risk from unintended side-effects such as exacerbation of HBV replication.
Used Model HepG2 and Huh7 cells
DOI 10.1111/jvh.12100