| Gene Name | LUC7L3 |
| HF Protein Name | Luc7-like protein 3 |
| HF Function | Negative regulator of virus ENII/BCP |
| Uniprot ID | O95232 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 51747 |
| Host Factor (HF) Name in Paper | LUC7L3 |
| Gene synonyms | CREAP1 CROP O48 |
| Ensemble Gene ID | ENSG00000108848 |
| Ensemble Transcript | ENST00000240304 [O95232-1];ENST00000505658 [O95232-1] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0003677, GO:0003723, GO:0003729, GO:0005634, GO:0005654, GO:0005685, GO:0006376, GO:0008380, GO:0016607, GO:0071004, |
| MINT ID | O95232 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 609434 |
| PANTHER ID | PTHR12375 |
| PDB ID(s) | N.A., |
| pfam ID | PF03194, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Hepatitis B virus |
| Virus Short Name | HBV |
| Order | Unassigned |
| Virus Family | Hepadnaviridae |
| Virus Subfamily | N.A. |
| Genus | Orthohepadnavirus |
| Species | Hepatitis B virus |
| Host | Human, mammals |
| Cell Tropism | Hepatocytes |
| Associated Disease | Hepatitis, hepatocellular carcinoma(chronic infections), cirrhosis |
| Mode of Transmission | Sexual contact, blood, maternal-neonatal |
| VIPR DB link | N.A. |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/reverse-transcribing-dna-and-rna-viruses-2011/w/rt_viruses/155/hepadnaviridae |
| Virus Host DB link | N.A. |
| Paper Title | LUC7L3/CROP inhibits replication of hepatitis B virus via suppressing enhancer II/basal core promoter activity |
| Author's Name | Yuan Li, Masahiko Ito, Suofeng Sun, TakeshiChida, Kenji Nakashima & Tetsuro Suzuki |
| Journal Name | Scientific Reports |
| Pubmed ID | 27857158 |
| Abstract | The core promoter of hepatitis B virus (HBV) genome is a critical region for transcriptional initiation of 3.5 kb, pregenome and precore RNAs and for the viral replication. Although a number of host-cell factors that potentially regulate the viral promoter activities have been identified, the molecular mechanisms of the viral gene expression, in particular, regulatory mechanisms of the transcriptional repression remain elusive. In this study, we identified LUC7 like 3 pre-mRNA splicing factor (LUC7L3, also known as hLuc7A or CROP) as a novel interacting partner of HBV enhancer II and basal core promoter (ENII/BCP), key elements within the core promoter, through the proteomic screening and found that LUC7L3 functions as a negative regulator of ENII/BCP. Gene silencing of LUC7L3 significantly increased expression of the viral genes and antigens as well as the activities of ENII/BCP and core promoter. In contrast, overexpression of LUC7L3 inhibited their activities and HBV replication. In addition, LUC7L3 possibly contributes to promotion of the splicing of 3.5 kb RNA, which may also be involved in negative regulation of the pregenome RNA level. This is the first to demonstrate the involvement of LUC7L3 in regulation of gene transcription and in viral replication. |
| Used Model | HuH7 cells |
| DOI | 10.1038/srep36741 |
