| Gene Name | KDM8 |
| HF Protein Name | JmjC domain-containing protein 5 |
| HF Function | Direct interaction of JMJD5 with HBx facilitates HBV replication |
| Uniprot ID | Q8N371 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 79831 |
| Host Factor (HF) Name in Paper | JMJD5 |
| Gene synonyms | JMJD5 |
| Ensemble Gene ID | ENSG00000155666 |
| Ensemble Transcript | ENST00000286096 [Q8N371-1];ENST00000441782 [Q8N371-3];ENST00000568965 [Q8N371-2] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000086, GO:0003682, GO:0005634, GO:0005654, GO:0005694, GO:0005829, GO:0006351, GO:0008233, GO:0032452, GO:0045893, GO:0046872, GO:0051864, GO:0070544, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 611917 |
| PANTHER ID | N.A. |
| PDB ID(s) | 3UYJ, 4AAP, 4GAZ, 4GJY, 4GJZ, 4QU1, 5FBJ, 6AVS, 6AX3, |
| pfam ID | N.A., |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Hepatitis B virus |
| Virus Short Name | HBV |
| Order | Unassigned |
| Virus Family | Hepadnaviridae |
| Virus Subfamily | N.A. |
| Genus | Orthohepadnavirus |
| Species | Hepatitis B virus |
| Host | Human, mammals |
| Cell Tropism | Hepatocytes |
| Associated Disease | Hepatitis, hepatocellular carcinoma(chronic infections), cirrhosis |
| Mode of Transmission | Sexual contact, blood, maternal-neonatal |
| VIPR DB link | N.A. |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/reverse-transcribing-dna-and-rna-viruses-2011/w/rt_viruses/155/hepadnaviridae |
| Virus Host DB link | N.A. |
| Paper Title | Hepatocyte Factor JMJD5 Regulates Hepatitis B Virus Replication through Interaction with HBx |
| Author's Name | Takahisa Kouwaki, Toru Okamoto, Ayano Ito, Yukari Sugiyama, Kazuo Yamashita, Tatsuya Suzuki, Shinji Kusakabe, Junki Hirano, Takasuke Fukuhara, Atsuya Yamashita, Kazunobu Saito, Daisuke Okuzaki, Koichi Watashi, Masaya Sugiyama, Sachiyo Yoshio, Daron M. Standley, Tatsuya Kanto, Masashi Mizokami, Kohji Moriishi, Yoshiharu Matsuura |
| Journal Name | Journal Of Virology |
| Pubmed ID | 26792738 |
| Abstract | Hepatitis B virus (HBV) is a causative agent for chronic liver diseases such as hepatitis, cirrhosis, and hepatocellular carcinoma (HCC). HBx protein encoded by the HBV genome plays crucial roles not only in pathogenesis but also in replication of HBV. Although HBx has been shown to bind to a number of host proteins, the molecular mechanisms by which HBx regulates HBV replication are largely unknown. In this study, we identified jumonji C-domain-containing 5 (JMJD5) as a novel binding partner of HBx interacting in the cytoplasm. DNA microarray analysis revealed that JMJD5-knockout (JMJD5KO) Huh7 cells exhibited a significant reduction in the expression of transcriptional factors involved in hepatocyte differentiation, such as HNF4A, CEBPA, and FOXA3. We found that hydroxylase activity of JMJD5 participates in the regulation of these transcriptional factors. Moreover, JMJD5KO Huh7 cells exhibited a severe reduction in HBV replication, and complementation of HBx expression failed to rescue replication of a mutant HBV deficient in HBx, suggesting that JMJD5 participates in HBV replication through an interaction with HBx. We also found that replacing Gly(135) with Glu in JMJD5 abrogates binding with HBx and replication of HBV. Moreover, the hydroxylase activity of JMJD5 was crucial for HBV replication. Collectively, these results suggest that direct interaction of JMJD5 with HBx facilitates HBV replication through the hydroxylase activity of JMJD5. |
| Used Model | HEK293T, Huh7, HepG2, HepG2.2.15-7 cells |
| DOI | 10.1128/JVI.02776-15 |
