| Gene Name | ASGR1 |
| HF Protein Name | Asialoglycoprotein receptor 1 |
| HF Function | Cellular receptor for HEV virus |
| Uniprot ID | P07306 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 432 |
| Host Factor (HF) Name in Paper | ASGR1 |
| Gene synonyms | CLEC4H1 |
| Ensemble Gene ID | ENSG00000141505 |
| Ensemble Transcript | ENST00000269299 [P07306-1];ENST00000574388 [P07306-2];ENST00000619926 [P07306-2] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0004873, GO:0005576, GO:0005886, GO:0005887, GO:0006898, GO:0018279, GO:0030246, GO:0031668, GO:0042803, GO:0046872, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 108360 |
| PANTHER ID | N.A. |
| PDB ID(s) | 1DV8, 5JPV, 5JQ1, |
| pfam ID | PF00059, PF03954, |
| Drug Bank ID | N.A., |
| ChEMBL ID | CHEMBL2084 |
| Organism | Homo sapiens (Human) |
| Virus Name | Hepatitis E virus |
| Virus Short Name | HEV |
| Order | Unassigned |
| Virus Family | Hepeviridae |
| Virus Subfamily | N.A. |
| Genus | Orthohepevirus |
| Species | Hepatitis E virus |
| Host | Human, pig, wild boar, monkey, some rodents, chicken |
| Cell Tropism | Intestinal tract, hepatocytes and cells in the biliary tract |
| Associated Disease | Hepatitis |
| Mode of Transmission | Zoonosis, fomite |
| VIPR DB link | https://www.viprbrc.org/brc/aboutPathogen.spg?decorator=hepe |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/259/hepeviridae |
| Virus Host DB link | N.A. |
| Paper Title | Asialoglycoprotein Receptor Facilitates Infection of PLC/PRF/5 Cells by HEV Through Interaction With ORF2 |
| Author's Name | Li Zhang, Yabin Tian, Zhiheng Wen, Feng Zhang, Ying Qi, Weijin Huang, Heqiu Zhang and Youchun Wang |
| Journal Name | Journal of Medical Virology |
| Pubmed ID | 27155063 |
| Abstract | Although the biological and epidemiological features of hepatitis E virus (HEV) have been studied extensively in recent years, the mechanism by which HEV infects cells is still poorly understood. In this study, coimmunoprecipitation, pull-down, and ELISA were used to show that the HEV ORF2 protein interacts directly with the ectodomain of both ASGR1 and ASGR2. Susceptibility to HEV correlated positively with the expression level of surface asialoglycoprotein receptor (ASGPR) in cell lines. ASGPR-directed small interfering RNA (siRNA) in HEV-infected PLC/PRF/5 cells had no significant effect on HEV release, suggesting that ASGPR mainly regulates the viral binding and entry steps. Both the purified ASGPR ectodomain and anti-ASGPR antibodies disturbed the binding of HEV to PLC/PRF/5cells. The classic ASGPR ligands asialofetuin, asialoganglioside, and fibronectin competitively inhibited the binding of HEV to hepatocytes in the presence of calcium. HeLa cell lines stably expressing ASGPR displayed increased HEV-binding capacity, whereas ASGPR-knockout PLC/PRF/5 cell lines had lower HEV-binding capacity. Thus, our study demonstrates that ASGPR is involved in and facilitates HEV infection by binding to ORF2. |
| Used Model | HeLa, A549 and HepG2 cells |
| DOI | 10.1002/jmv.24570 |
