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Virus Details


VHFID3820

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name GBF1
HF Protein Name Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
HF Function Required for hepatitis E virus RNA replication
Uniprot ID Q92538
Protein Sequence View Fasta Sequence
NCBI Gene ID 8729
Host Factor (HF) Name in Paper GBF1
Gene synonyms KIAA0248
Ensemble Gene ID ENSG00000107862
Ensemble Transcript ENST00000369983 [Q92538-1]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000139, GO:0002263, GO:0005085, GO:0005086, GO:0005547, GO:0005739, GO:0005777, GO:0005788, GO:0005793, GO:0005794, GO:0005795, GO:0005801, GO:0005802, GO:0005811, GO:0005829, GO:0006888, GO:0006890, GO:0006892, GO:0006895, GO:0007030, GO:0007346, GO:0015031, GO:0016020, GO:0016032, GO:0030593, GO:0031252, GO:0032012, GO:0034067, GO:0042147, GO:0048205, GO:0061162, GO:0070973, GO:0080025, GO:0090166, GO:0097111, GO:0098586, GO:1903409, GO:1903420, GO:2000008,
MINT ID Q92538
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 603698
PANTHER ID N.A.
PDB ID(s) N.A.,
pfam ID PF01369, PF12783,
Drug Bank ID N.A.,
ChEMBL ID N.A.
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Hepatitis E virus
Virus Short Name HEV
Order Unassigned
Virus Family Hepeviridae
Virus Subfamily N.A.
Genus Orthohepevirus
Species Hepatitis E virus
Host Human, pig, wild boar, monkey, some rodents, chicken
Cell Tropism Intestinal tract, hepatocytes and cells in the biliary tract
Associated Disease Hepatitis
Mode of Transmission Zoonosis, fomite
VIPR DB link https://www.viprbrc.org/brc/aboutPathogen.spg?decorator=hepe
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/259/hepeviridae
Virus Host DB link N.A.

Publication Information

Paper Title Identification of GBF1 as a cellular factor required for hepatitis E virus RNA replication
Author's Name Rayan Farhat, Maliki Ankavay, Nadjet Lebsir, Jer me Gouttenoire, Catherine L. Jackson, Czeslaw Wychowski, Darius Moradpour, Jean Dubuisson, Yves Rouille, Laurence Cocquerel
Journal Name cellular microbiology
Pubmed ID 29112323
Abstract The hepatitis E virus (HEV) genome is a single-stranded, positive-sense RNA that encodes three proteins including the ORF1 replicase. Mechanisms of HEV replication in host cells are unclear, and only a few cellular factors involved in this step have been identified so far. Here, we used brefeldin A (BFA) that blocks the activity of the cellular Arf guanine nucleotide exchange factors GBF1, BIG1, and BIG2, which play a major role in reshuffling of cellular membranes. We showed that BFA inhibits HEV replication in a dose-dependent manner. The use of siRNA and Golgicide A identified GBF1 as a host factor critically involved in HEV replication. Experiments using cells expressing a mutation in the catalytic domain of GBF1 and overexpression of wild type GBF1 or a BFA-resistant GBF1 mutant rescuing HEV replication in BFA-treated cells, confirmed that GBF1 is the only BFA-sensitive factor required for HEV replication. We demonstrated that GBF1 is likely required for the activity of HEV replication complexes. However, GBF1 does not colocalise with the ORF1 protein, and its subcellular distribution is unmodified upon infection or overexpression of viral proteins, indicating that GBF1 is likely not recruited to replication sites. Together, our results suggest that HEV replication involves GBF1-regulated mechanisms.
Used Model Huh7.5 cells
DOI 10.1111/cmi.12804