Virus Details


VHFID3961

Host Factor Information

Gene Name PPIA
HF Protein Name Peptidyl-prolyl cis-trans isomerase A
HF Function Essential for virus DNA replication and reactivation
Uniprot ID P62937
Protein Sequence View Fasta Sequence
NCBI Gene ID 5478
Host Factor (HF) Name in Paper CyPA
Gene synonyms CYPA
Ensemble Gene ID ENSG00000196262
Ensemble Transcript ENST00000355968 [P62937-2];ENST00000468812 [P62937-1];ENST00000489459 [P62937-2];ENST00000620047 [P62937-2]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000413, GO:0003723, GO:0003755, GO:0005576, GO:0005615, GO:0005634, GO:0005829, GO:0005925, GO:0006278, GO:0006457, GO:0016018, GO:0016020, GO:0019058, GO:0019061, GO:0019064, GO:0019068, GO:0019076, GO:0030260, GO:0031982, GO:0032991, GO:0034389, GO:0034774, GO:0035722, GO:0043312, GO:0045069, GO:0045070, GO:0046790, GO:0050714, GO:0050900, GO:0051082, GO:0070062, GO:0075713, GO:1904813,
MINT ID P62937
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 123840
PANTHER ID PTHR11071
PDB ID(s) 1AK4, 1AWQ, 1AWR, 1AWS, 1AWT, 1AWU, 1AWV, 1BCK, 1CWA, 1CWB, 1CWC, 1CWF, 1CWH, 1CWI, 1CWJ, 1CWK, 1CWL, 1CWM, 1CWO, 1FGL, 1M63, 1M9C, 1M9D, 1M9E, 1M9F, 1M9X, 1M9Y, 1MF8, 1MIK, 1NMK, 1OCA, 1RMH, 1VBS, 1VBT, 1W8L, 1W8M, 1W8V, 1YND, 1ZKF, 2ALF, 2CPL, 2CYH, 2MS4, 2MZU, 2N0T, 2RMA, 2RMB, 2X25, 2X2A, 2X2C, 2X2D, 2XGY, 3CYH, 3CYS, 3K0M, 3K0N, 3K0O, 3K0P, 3K0Q, 3K0R, 3ODI, 3ODL, 3RDD, 4CYH, 4IPZ, 4N1M, 4N1N, 4N1O, 4N1P, 4N1Q, 4N1R, 4N1S, 4YUG, 4YUH, 4YUI, 4YUJ, 4YUK, 4YUL, 4YUM, 4YUN, 4YUO, 4YUP, 5CYH, 5F66, 5FJB, 5KUL, 5KUN, 5KUO, 5KUQ, 5KUR, 5KUS, 5KUU, 5KUV, 5KUW, 5KUZ, 5KV0, 5KV1, 5KV2, 5KV3, 5KV4, 5KV5, 5KV6, 5KV7, 5LUD, 5NOQ, 5NOR, 5NOS, 5NOT, 5NOU, 5NOV, 5NOW, 5NOX, 5NOY, 5NOZ, 5T9U, 5T9W, 5T9Z, 5TA2, 5TA4,
pfam ID PF00160,
Drug Bank ID DB01742, DB00091, DB02419, DB00172,
ChEMBL ID CHEMBL1949
Organism Homo sapiens (Human)

Pathogen Information

Virus Name human cytomegalo virus
Virus Short Name HCMV
Order Herpesvirales
Virus Family Herpesviridae
Virus Subfamily Betaherpesvirinae
Genus Cytomegalovirus
Species Human cytomegalovirus
Host Human, monkeys
Cell Tropism Epithelial cells, endothelial cells, fibroblasts and smooth muscle cells are predominant targets, but infects also parenchymal cells and connective tissue cells of virtually any organ and various hematopoietic cell types
Associated Disease Mononucleosis, peumonias
Mode of Transmission Contact, urine, saliva
VIPR DB link http://www.viprbrc.org/brc/vipr_allSpecies_search.do?method=SubmitForm&decorator=herpes
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/91/herpesviridae
Virus Host DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Herpesviridae

Publication Information

Paper Title Cyclophilin A is required for efficient human cytomegalovirus DNA replication and reactivation
Author's Name Lisa R. Keyes, Mariana G. Bego, Melisa Soland and Stephen St Jeor
Journal Name Journal Of General Virology
Pubmed ID 22238232
Abstract Human cytomegalovirus (HCMV) is a large DNA virus belonging to the subfamily Betaherpesvirinae. Haematopoietic cells of the myeloid lineage have been shown to harbour latent HCMV. However, following terminal differentiation of these cells, virus is reactivated, and in an immunocompromised host acute infection can occur. It is currently unknown which viral and cellular factors are involved in regulating the switch between lytic and latent infections. Cyclophilin A (CyPA) is a cellular protein that acts as a major factor in virus replication and/or virion maturation for a number of different viruses, including human immunodeficiency virus, hepatitis C virus, murine cytomegalovirus, influenza A virus and vaccinia virus. This study investigated the role of CyPA during HCMV infection. CyPA expression was silenced in human foreskin fibroblast (HF) and THP-1 cells using small interfering RNA (siRNA) technology, or the cells were treated with cyclosporin A (CsA) to inhibit CyPA activity. Silencing CyPA in HF cells with siRNA resulted in an overall reduction in virus production characterized by delayed expression of immediate-early (IE) proteins, decreased viral DNA loads and reduced titres. Furthermore, silencing of CyPA in THP-1 cells pre- and post-differentiation prevented IE protein expression and virus reactivation from a non-productive state. Interestingly, it was observed that treatment of THP-1 cells with CsA prevented the cells from establishing a fully latent infection. In summary, these results demonstrate that CyPA expression is an important factor in HCMV IE protein expression and virus production in lytically infected HF cells, and is a major component in virus reactivation from infected THP-1 cells.
Used Model THP-1 cells
DOI 10.1099/vir.0.037309-0