| Gene Name | CTCF |
| HF Protein Name | Transcriptional repressor CTCF |
| HF Function | It is a insulator protein which helps virus to maintain viral latency |
| Uniprot ID | P49711 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 10664 |
| Host Factor (HF) Name in Paper | CTCF |
| Gene synonyms | N.A. |
| Ensemble Gene ID | ENSG00000102974 |
| Ensemble Transcript | ENST00000264010 [P49711-1];ENST00000401394 [P49711-2] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000122, GO:0000775, GO:0000793, GO:0000978, GO:0000981, GO:0001078, GO:0003700, GO:0003714, GO:0005634, GO:0005654, GO:0005730, GO:0006351, GO:0007059, GO:0008270, GO:0008285, GO:0010628, GO:0016584, GO:0040029, GO:0040030, GO:0043035, GO:0043565, GO:0044212, GO:0045892, GO:0045893, GO:0070602, GO:0071459, |
| MINT ID | P49711 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 604167 |
| PANTHER ID | N.A. |
| PDB ID(s) | 1X6H, 2CT1, 5K5H, 5K5I, 5K5J, 5K5L, 5KKQ, 5T00, 5T0U, 5UND, 5YEF, 5YEG, 5YEH, 5YEL, |
| pfam ID | N.A., |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Human herpesvirus 4 (Epstein-Barr virus) |
| Virus Short Name | EBV |
| Order | Herpesvirales |
| Virus Family | Herpesviridae |
| Virus Subfamily | Gammaherpesvirinae |
| Genus | Lymphocryptovirus |
| Species | Human herpesvirus 4 |
| Host | Human, mammals |
| Cell Tropism | B lymphocytes, oral epithelial cells, latency: remains latent in cd19+ b cells |
| Associated Disease | Mononucleosis, associated with environemental diseases: burkitt?s lymphoma nasopharyngeal carcinoma (npc) |
| Mode of Transmission | Contact, saliva |
| VIPR DB link | http://www.viprbrc.org/brc/vipr_allSpecies_search.do?method=SubmitForm&decorator=herpes |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/91/herpesviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Herpesviridae |
| Paper Title | PARP1 restricts Epstein Barr Virus lytic reactivation by binding the BZLF1 promoter |
| Author's Name | Lena N. Lupey-Green, Stephanie A. Moquin, Kayla A. Martin, Shane M. McDevitt, Michael Hulse, Lisa B. Caruso, Richard T. Pomerantz, JJ L. Miranda and Italo Tempera |
| Journal Name | Virology |
| Pubmed ID | 28456021 |
| Abstract | The Epstein Barr virus (EBV) genome persists in infected host cells as a chromatinized episome and is subject to chromatin-mediated regulation. Binding of the host insulator protein CTCF to the EBV genome has an established role in maintaining viral latency type, and in other herpesviruses, loss of CTCF binding at specific regions correlates with viral reactivation. Here, we demonstrate that binding of PARP1, an important cofactor of CTCF, at the BZLF1 lytic switch promoter restricts EBV reactivation. Knockdown of PARP1 in the Akata-EBV cell line significantly increases viral copy number and lytic protein expression. Interestingly, CTCF knockdown has no effect on viral reactivation, and CTCF binding across the EBV genome is largely unchanged following reactivation. Moreover, EBV reactivation attenuates PARP activity, and Zta expression alone is sufficient to decrease PARP activity. Here we demonstrate a restrictive function of PARP1 in EBV lytic reactivation. |
| Used Model | HEK293 cells |
| DOI | 10.1016/j.virol.2017.04.006 |
