| Gene Name | SRSF3 |
| HF Protein Name | Serine/arginine-rich splicing factor 3 |
| HF Function | SM modulates splice site selection of the host cell STAT1 pre-mRNA by using SRp20 |
| Uniprot ID | P84103 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 6428 |
| Host Factor (HF) Name in Paper | SRP20 |
| Gene synonyms | SFRS3 SRP20 |
| Ensemble Gene ID | ENSG00000112081 |
| Ensemble Transcript | ENST00000373715 [P84103-1];ENST00000477442 [P84103-2];ENST00000620941 [P84103-1] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000398, GO:0003723, GO:0005654, GO:0005737, GO:0006369, GO:0006405, GO:0006406, GO:0016607, GO:0031124, GO:0043274, GO:0048024, GO:1990825, GO:1990830, |
| MINT ID | P84103 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 603364 |
| PANTHER ID | N.A. |
| PDB ID(s) | 2I2Y, 2I38, |
| pfam ID | PF00076, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Human herpesvirus 4 (Epstein-Barr virus) |
| Virus Short Name | EBV |
| Order | Herpesvirales |
| Virus Family | Herpesviridae |
| Virus Subfamily | Gammaherpesvirinae |
| Genus | Lymphocryptovirus |
| Species | Human herpesvirus 4 |
| Host | Human, mammals |
| Cell Tropism | B lymphocytes, oral epithelial cells, latency: remains latent in cd19+ b cells |
| Associated Disease | Mononucleosis, associated with environemental diseases: burkitt?s lymphoma nasopharyngeal carcinoma (npc) |
| Mode of Transmission | Contact, saliva |
| VIPR DB link | http://www.viprbrc.org/brc/vipr_allSpecies_search.do?method=SubmitForm&decorator=herpes |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/91/herpesviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Herpesviridae |
| Paper Title | Epstein-Barr Virus SM Protein Utilizes Cellular Splicing Factor SRp20 To Mediate Alternative Splicing |
| Author's Name | Dinesh Verma, Swarna Bais, Melusine Gaillard, and Sankar Swaminathan |
| Journal Name | Journal Of Virology |
| Pubmed ID | 20810723 |
| Abstract | Epstein-Barr virus (EBV) SM protein is an essential nuclear protein produced during the lytic cycle of EBV replication. SM is an RNA-binding protein with multiple mechanisms of action. SM enhances the expression of EBV genes by stabilizing mRNA and facilitating nuclear export. SM also influences splicing of both EBV and cellular pre-mRNAs. SM modulates splice site selection of the host cell STAT1 pre-mRNA, directing utilization of a novel 5 splice site that is used only in the presence of SM. SM activates splicing in the manner of SR proteins but does not contain the canonical RS domains typical of cellular splicing factors. Affinity purification and mass spectrometry of SM complexes from SM-transfected cells led to the identification of the cellular SR splicing factor SRp20 as an SM-interacting protein. The regions of SM and SRp20 required for interaction were mapped by in vitro and in vivo assays. The SRp20 interaction was shown to be important for the effects of SM on alternative splicing by the use of STAT1 splicing assays. Overexpression of SRp20 enhanced SM-mediated alternative splicing and knockdown of SRp20 inhibited the SM effect on splicing. These data suggest a model whereby SM, a viral protein, recruits and co-opts the function of cellular SRp20 in alternative splicing. |
| Used Model | 293T and HeLa cells |
| DOI | 10.1128/JVI.01359-10 |
