| Gene Name | TRIM5 |
| HF Protein Name | Tripartite motif-containing protein 5 |
| HF Function | Inhibits viral replication |
| Uniprot ID | Q9C035 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 85363 |
| Host Factor (HF) Name in Paper | TRIM5alpha |
| Gene synonyms | RNF88 |
| Ensemble Gene ID | ENSG00000132256 |
| Ensemble Transcript | ENST00000380027 [Q9C035-4];ENST00000380034 [Q9C035-1];ENST00000396847 [Q9C035-3];ENST00000433961 [Q9C035-5] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000932, GO:0002218, GO:0004842, GO:0005634, GO:0005737, GO:0005829, GO:0006914, GO:0008270, GO:0008329, GO:0016032, GO:0019901, GO:0030674, GO:0031664, GO:0032880, GO:0042802, GO:0042803, GO:0043123, GO:0043410, GO:0045087, GO:0046597, GO:0051091, GO:0051092, GO:0051607, GO:0060333, GO:0070206, GO:0070534, GO:1902187, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 608487 |
| PANTHER ID | PTHR24103:SF426 |
| PDB ID(s) | 2ECV, 2YRG, |
| pfam ID | PF00622, PF00643, PF13445, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Human herpesvirus 4 (Epstein-Barr virus) |
| Virus Short Name | EBV |
| Order | Herpesvirales |
| Virus Family | Herpesviridae |
| Virus Subfamily | Gammaherpesvirinae |
| Genus | Lymphocryptovirus |
| Species | Human herpesvirus 4 |
| Host | Human, mammals |
| Cell Tropism | B lymphocytes, oral epithelial cells, latency: remains latent in cd19+ b cells |
| Associated Disease | Mononucleosis, associated with environemental diseases: burkitt?s lymphoma nasopharyngeal carcinoma (npc) |
| Mode of Transmission | Contact, saliva |
| VIPR DB link | http://www.viprbrc.org/brc/vipr_allSpecies_search.do?method=SubmitForm&decorator=herpes |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/91/herpesviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Herpesviridae |
| Paper Title | TRIM5alpha Promotes Ubiquitination of Rta from Epstein?Barr Virus to Attenuate Lytic Progression |
| Author's Name | Hsiang-Hung Huang, Chien-Sin Chen, Wen-Hung Wang, Shih-Wei Hsu, Hsiao-Han Tsai, Shih-Tung Liu and Li-Kwan Chang |
| Journal Name | Frontiers in Microbiology |
| Pubmed ID | 28105027 |
| Abstract | Replication and transcription activator (Rta), a key protein expressed by Epstein-Barr virus (EBV) during the immediate-early stage of the lytic cycle, is responsible for the activation of viral lytic genes. In this study, GST-pulldown and coimmunoprecipitation assays showed that Rta interacts in vitro and in vivo with TRIM5alpha, a host factor known to be involved in the restriction of retroviral infections. Confocal microscopy results revealed that Rta colocalizes with TRIM5alpha in the nucleus during lytic progression. The interaction involves 190 amino acids in the N-terminal of Rta and the RING domain in TRIM5alpha, and it was further found that TRIM5alpha acts as an E3 ubiquitin ligase to promote Rta ubiquitination. Overexpression of TRIM5alpha reduced the transactivating capabilities of Rta, while reducing TRIM5alpha expression enhanced EBV lytic protein expression and DNA replication. Taken together, these results point to a critical role for TRIM5alpha in attenuating EBV lytic progression through the targeting of Rta for ubiquitination, and suggest that the restrictive capabilities of TRIM5alpha may go beyond retroviral infections. |
| Used Model | P3HR1and 293T cells |
| DOI | 10.3389/fmicb.2016.02129 |
