| Virus Name | Human immunodeficiency virus 1 |
| Virus Short Name | HIV1 |
| Order | Unassigned |
| Virus Family | Retroviridae |
| Virus Subfamily | Orthoretrovirinae |
| Genus | Lentivirus |
| Species | Human immunodeficiency virus 1 |
| Host | Vertebrates |
| Cell Tropism | CD4+ T cells, macrophages and dendritic cells |
| Associated Disease | Acquired immunodeficiency syndrome |
| Mode of Transmission | Sexual contact, blood, breast feeding |
| VIPR DB link | N.A. |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/reverse-transcribing-dna-and-rna-viruses-2011/w/rt_viruses/161/retroviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Retroviridae |
| Paper Title | Genome-scale RNAi screen for host factors required for HIV replication |
| Author's Name | Honglin Zhou, Min Xu, Qian Huang, Adam T. Gates, Xiaohua D. Zhang, John C. Castle, Erica Stec, Marc Ferrer, Berta Strulovici, Daria J. Hazuda, and Amy S. Espeseth |
| Journal Name | Cell Host and Microbe |
| Pubmed ID | 18976975 |
| Abstract | Human immunodeficiency virus (HIV)-1 depends on the host cell machinery to support its replication. To discover cellular factors associated with HIV-1 replication, we conducted a genome-scale siRNA screen, revealing more than 311 host factors, including 267 that were not previously linked to HIV. Surprisingly, there was little overlap between these genes and the HIV dependency factors described recently. However, an analysis of the genes identified in both screens revealed overlaps in several of the associated pathways or protein complexes, including the SP1/mediator complex and the NF-kappaB signaling pathway. cDNAs for a subset of the identified genes were used to rescue HIV replication following knockdown of the cellular mRNA providing strong evidence that the following six genes are previously uncharacterized host factors for HIV: AKT1, PRKAA1, CD97, NEIL3, BMP2K, and SERPINB6. This study highlights both the power and shortcomings of large scale loss-of-function screens in discovering host-pathogen interactions. |
| Used Model | HeLa cells |
| DOI | 10.1016/j.chom.2008.10.004 |
