| Gene Name | TRAPPC8 |
| HF Protein Name | Trafficking protein particle complex subunit 8 |
| HF Function | Required for Human Papillomavirus cell entry |
| Uniprot ID | Q9Y2L5 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 22878 |
| Host Factor (HF) Name in Paper | TRAPPC8 |
| Gene synonyms | KIAA1012 |
| Ensemble Gene ID | ENSG00000153339 |
| Ensemble Transcript | ENST00000283351 [Q9Y2L5-1] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000407, GO:0005829, GO:0006888, GO:0007030, GO:0017112, GO:0030008, GO:0030242, GO:0031410, GO:0034497, GO:0044804, GO:1990072, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 614136 |
| PANTHER ID | PTHR12975 |
| PDB ID(s) | N.A., |
| pfam ID | PF12739, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Human papillomavirus type 16 |
| Virus Short Name | HPV16 |
| Order | Unassigned |
| Virus Family | Papillomaviridae |
| Virus Subfamily | N.A. |
| Genus | Alphapapillomavirus |
| Species | Human papillomavirus 16 |
| Host | Human, monkeys |
| Cell Tropism | Epithelial cells of skin, mucous membranes |
| Associated Disease | Malignant tumours |
| Mode of Transmission | Sexual, indirect and direct contact, auto-inoculation |
| VIPR DB link | N.A. |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/121/papillomaviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Papillomaviridae |
| Paper Title | Identification of TRAPPC8 as a host factor required for human papillomavirus cell entry |
| Author's Name | Yoshiyuki Ishii, Tomomi Nakahara, Michiyo Kataoka, Rika Kusumoto-Matsuo, Seiichiro Mori, Takamasa Takeuchi, Iwao Kukimoto |
| Journal Name | PLOS One |
| Pubmed ID | 24244674 |
| Abstract | Human papillomavirus (HPV) is a non-enveloped virus composed of a circular DNA genome and two capsid proteins, L1 and L2. Multiple interactions between its capsid proteins and host cellular proteins are required for infectious HPV entry, including cell attachment and internalization, intracellular trafficking and viral genome transfer into the nucleus. Using two variants of HPV type 51, the Ma and Nu strains, we have previously reported that MaL2 is required for efficient pseudovirus (PsV) transduction. However, the cellular factors that confer this L2 dependency have not yet been identified. Here we report that the transport protein particle complex subunit 8 (TRAPPC8) specifically interacts with MaL2. TRAPPC8 knockdown in HeLa cells yielded reduced levels of reporter gene expression when inoculated with HPV51Ma, HPV16, and HPV31 PsVs. TRAPPC8 knockdown in HaCaT cells also showed reduced susceptibility to infection with authentic HPV31 virions, indicating that TRAPPC8 plays a crucial role in native HPV infection. Immunofluorescence microscopy revealed that the central region of TRAPPC8 was exposed on the cell surface and colocalized with inoculated PsVs. The entry of Ma, Nu, and L2-lacking PsVs into cells was equally impaired in TRAPPC8 knockdown HeLa cells, suggesting that TRAPPC8-dependent endocytosis plays an important role in HPV entry that is independent of L2 interaction. Finally, expression of GFP-fused L2 that can also interact with TRAPPC8 induced dispersal of the Golgi stack structure in HeLa cells, a phenotype also observed by TRAPPC8 knockdown. These results suggest that during viral intracellular trafficking, binding of L2 to TRAPPC8 inhibits its function resulting in Golgi destabilization, a process that may assist HPV genome escape from the trans-Golgi network. |
| Used Model | HeLa, HaCaT and HEK293FT cells |
| DOI | 10.1371/journal.pone.0080297 |
