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Virus Details


VHFID4975

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name SP100
HF Protein Name Nuclear autoantigen Sp-100
HF Function Host Restriction factor
Uniprot ID P23497
Protein Sequence View Fasta Sequence
NCBI Gene ID 6672
Host Factor (HF) Name in Paper Sp100
Gene synonyms N.A.
Ensemble Gene ID ENSG00000067066
Ensemble Transcript ENST00000264052 [P23497-1];ENST00000340126 [P23497-4];ENST00000409112 [P23497-3];ENST00000409341 [P23497-2];ENST00000409897 [P23497-7];ENST00000427101 [P23497-6]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000122, GO:0000723, GO:0000784, GO:0000981, GO:0003677, GO:0003713, GO:0003714, GO:0005634, GO:0005654, GO:0005730, GO:0005737, GO:0006351, GO:0006978, GO:0008134, GO:0010596, GO:0016032, GO:0016604, GO:0016605, GO:0019900, GO:0019904, GO:0032526, GO:0032897, GO:0034097, GO:0034340, GO:0034341, GO:0034399, GO:0042802, GO:0042803, GO:0043392, GO:0043433, GO:0045185, GO:0045765, GO:0045892, GO:0045893, GO:0046826, GO:0048384, GO:0051091, GO:0051271, GO:0060333, GO:0060337, GO:0070087, GO:1902041, GO:1902044,
MINT ID P23497
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 604585
PANTHER ID N.A.
PDB ID(s) 1H5P, 4PTB, 5FB0, 5FB1, 5PWE, 5PWF, 5PWG, 5PWH, 5PWI, 5PWJ, 5PWK, 5PWL, 5PWM, 5PWN, 5PWO, 5PWP, 5PWQ, 5PWR, 5PWS, 5PWT, 5PWU, 5PWV, 5PWW, 5PWX, 5PWY, 5PWZ, 5PX0, 5PX1, 5PX2, 5PX3, 5PX4, 5PX5, 5PX6, 5PX7, 5PX8, 5PX9, 5PXA, 5PXB, 5PXC, 5PXD, 5PXE, 5PXF, 5PXG, 5PXH, 5PXI, 5PXJ, 5PXK, 5PXL, 5PXM, 5PXN, 5PXO, 5PXP, 5PXQ, 5PXR, 5PXS, 5PXT, 5PXU, 5PXV, 5PXW, 5PXX, 5PXY, 5PXZ, 5PY0, 5PY1, 5PY2, 5PY3, 5PY4, 5PY5, 5PY6, 5PY7, 5PY8, 5PY9, 5PYA, 5PYB, 5PYC, 5PYD, 5PYE, 5PYF, 5PYG, 5PYH, 5PYI, 5PYJ, 5PYK, 5PYL, 5PYM, 5PYN, 5PYO, 5PYP, 5PYQ, 5PYR, 5PYS, 5PYT, 5PYU, 5PYV, 5PYW, 5PYX, 5PYY, 5PYZ, 5PZ0, 5PZ1, 5PZ2, 5PZ3, 5PZ4, 5PZ5, 5PZ6, 5PZ7, 5PZ8, 5PZ9, 5PZA, 5PZB, 5PZC, 5PZD, 5PZE, 5PZF, 5PZG, 5PZH, 5PZI, 5PZJ,
pfam ID PF00505, PF09011, PF03172, PF01342,
Drug Bank ID N.A.,
ChEMBL ID N.A.
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Human papillomavirus type 16
Virus Short Name HPV16
Order Unassigned
Virus Family Papillomaviridae
Virus Subfamily N.A.
Genus Alphapapillomavirus
Species Human papillomavirus 16
Host Human, monkeys
Cell Tropism Epithelial cells of skin, mucous membranes
Associated Disease Malignant tumours
Mode of Transmission Sexual, indirect and direct contact, auto-inoculation
VIPR DB link N.A.
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/121/papillomaviridae
Virus Host DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Papillomaviridae

Publication Information

Paper Title Interferon Kappa Inhibits Human Papillomavirus 31 Transcription by Inducing Sp100 Proteins
Author's Name Christina Habiger, Gunter Jager, Michael Walter, Thomas Iftner, Frank Stubenrauch
Journal Name Journal Of Virology
Pubmed ID 26491169
Abstract High-risk human papillomaviruses (hr-HPV) establish persistent infections in keratinocytes, which can lead to cancer of the anogenital tract. Interferons (IFNs) are a family of secreted cytokines that induce IFN-stimulated genes (ISGs), many of which display antiviral activities. Transcriptome studies have indicated that established hr-HPV-positive cell lines display a reduced expression of ISGs, which correlates with decreased levels of interferon kappa (IFN-κ), a type I IFN constitutively expressed in keratinocytes. Prior studies have also suggested that IFN-beta has anti-hr-HPV activity but the underlying mechanisms are not well understood. The downregulation of IFN-κ by hr-HPV raises the possibility that IFN-κ has anti-HPV activity. Using doxycycline-inducible IFN-κ expression in CIN612-9E cells, which maintain extrachromosomally replicating HPV31 genomes, we demonstrated that IFN-κ inhibits the growth of these cells and reduces viral transcription and replication. Interestingly, the initiation of viral early transcription was already inhibited at 4 to 6 h after IFN-κ expression. This was also observed with recombinant IFN-beta, suggesting a common mechanism of IFNs. Transcriptome sequencing (RNA-seq) analysis identified 1,367 IFN-κ-regulated genes, of which 221 were modulated >2-fold. The majority of those (71%) matched known ISGs, confirming that IFN-κ acts as a bona fide type I IFN in hr-HPV-positive keratinocytes. RNA interference (RNAi) and cotransfection experiments indicated that the inhibition of viral transcription is mainly due to the induction of Sp100 proteins by IFN-κ. Consistent with published data showing that Sp100 acts as a restriction factor for HPV18 infection, our results suggest that hr-HPV target IFN-κ to prevent Sp100 expression and identify Sp100 as an ISG with anti-HPV activity.
Used Model CIN612-9E and NIH 3T3
DOI 10.1128/JVI.02137-15