| Gene Name | OBSL1 |
| HF Protein Name | Obscurin-like protein 1 |
| HF Function | Required for HPV16 endocytosis |
| Uniprot ID | O75147 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 23363 |
| Host Factor (HF) Name in Paper | OBSL1 |
| Gene synonyms | KIAA0657 |
| Ensemble Gene ID | ENSG00000124006 |
| Ensemble Transcript | ENST00000373873 [O75147-2];ENST00000404537 [O75147-3];ENST00000603926 [O75147-4] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000226, GO:0005737, GO:0005794, GO:0005813, GO:0005829, GO:0005859, GO:0006941, GO:0007010, GO:0007015, GO:0007030, GO:0007088, GO:0008093, GO:0008307, GO:0014704, GO:0030018, GO:0031430, GO:0034067, GO:0043687, GO:0045214, GO:0048471, GO:0050775, GO:0051015, GO:0051371, GO:0055003, GO:0071688, GO:0097493, GO:1990393, |
| MINT ID | O75147 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 610991 |
| PANTHER ID | N.A. |
| PDB ID(s) | 2CPC, 2E6P, 2E6Q, 2LU7, 2LVC, 2WP3, 2WWK, 2WWM, 3KNB, 5FM5, |
| pfam ID | PF07679, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Human papillomavirus type 16 |
| Virus Short Name | HPV16 |
| Order | Unassigned |
| Virus Family | Papillomaviridae |
| Virus Subfamily | N.A. |
| Genus | Alphapapillomavirus |
| Species | Human papillomavirus 16 |
| Host | Human, monkeys |
| Cell Tropism | Epithelial cells of skin, mucous membranes |
| Associated Disease | Malignant tumours |
| Mode of Transmission | Sexual, indirect and direct contact, auto-inoculation |
| VIPR DB link | N.A. |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/121/papillomaviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Papillomaviridae |
| Paper Title | The Cytoskeletal Adaptor Obscurin-Like 1 Interacts with the Human Papillomavirus 16 (HPV16) Capsid Protein L2 and Is Required for HPV16 Endocytosis |
| Author's Name | Elena Wustenhagen, Laura Hampe, Fatima Boukhallouk, Marc A. Schneider, Gilles A. Spoden, Inka Negwer, Kaloian Koynov, W. Martin Kast, Luise Florin |
| Journal Name | Journal Of Virology |
| Pubmed ID | 27654294 |
| Abstract | The human papillomavirus (HPV) capsid protein L2 is essential for viral entry. To gain a deeper understanding of the role of L2, we searched for novel cellular L2-interacting proteins. A yeast two-hybrid analysis uncovered the actin-depolymerizing factor gelsolin, the membrane glycoprotein dysadherin, the centrosomal protein 68 (Cep68), and the cytoskeletal adaptor protein obscurin-like 1 protein (OBSL1) as putative L2 binding molecules. Pseudovirus (PsV) infection assays identified OBSL1 as a host factor required for gene transduction by three oncogenic human papillomavirus types, HPV16, HPV18, and HPV31. In addition, we detected OBSL1 expression in cervical tissue sections and noted the involvement of OBSL1 during gene transduction of primary keratinocytes by HPV16 PsV. Complex formation of HPV16 L2 with OBSL1 was demonstrated in coimmunofluorescence and coimmunoprecipitation studies after overexpression of L2 or after PsV exposure. We observed a strong colocalization of OBSL1 with HPV16 PsV and tetraspanin CD151 at the plasma membrane, suggesting a role for OBSL1 in viral endocytosis. Indeed, viral entry assays exhibited a reduction of viral endocytosis in OBSL1-depleted cells. Our results suggest OBSL1 as a novel L2-interacting protein and endocytosis factor in HPV infection. |
| Used Model | HaCaT cells |
| DOI | 10.1128/JVI.01222-16 |
