| Gene Name | WDR46 |
| HF Protein Name | WD repeat-containing protein 46 |
| HF Function | Required for VSV infection |
| Uniprot ID | O15213 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 9277 |
| Host Factor (HF) Name in Paper | WDR46 |
| Gene synonyms | BING4 C6orf11 |
| Ensemble Gene ID | ENSG00000227057 |
| Ensemble Transcript | ENST00000374617;ENST00000383208;ENST00000432609;ENST00000432933;ENST00000457382 |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000462, GO:0003723, GO:0005654, GO:0005730, GO:0006364, GO:0030686, GO:0032040, GO:1903955, |
| MINT ID | O15213 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 611440 |
| PANTHER ID | N.A. |
| PDB ID(s) | N.A., |
| pfam ID | PF08149, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Vesicular stomatitis Indiana virus |
| Virus Short Name | VSV |
| Order | Mononegavirales |
| Virus Family | Rhabdoviridae |
| Virus Subfamily | N.A. |
| Genus | Vesiculovirus |
| Species | Indiana vesiculovirus |
| Host | Human, cattle, horse, swine, sandflies, blackflies |
| Cell Tropism | N.A. |
| Associated Disease | Vesicular diseases, encephalitis |
| Mode of Transmission | Mostely by sandflies |
| VIPR DB link | https://www.viprbrc.org/brc/vipr_allSpecies_search.spg?method=SubmitForm&decorator=rhabdo |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/201/rhabdoviridae |
| Virus Host DB link | N.A. |
| Paper Title | RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses |
| Author's Name | Debasis Panda, Anshuman Das, Phat X. Dinh, Sakthivel Subramaniam, Debasis Nayak, Nicholas J. Barrows, James L. Pearson, Jesse Thompson, David L. Kelly, Istvan Ladunga, and Asit K. Pattnaik |
| Journal Name | PNAS |
| Pubmed ID | 22065774 |
| Abstract | Negative-strand (NS) RNA viruses comprise many pathogens that cause serious diseases in humans and animals. Despite their clinical importance, little is known about the host factors required for their infection. Using vesicular stomatitis virus (VSV), a prototypic NS RNA virus in the family Rhabdoviridae, we conducted a human genome-wide siRNA screen and identified 72 host genes required for viral infection. Many of these identified genes were also required for infection by two other NS RNA viruses, the lymphocytic choriomeningitis virus of the Arenaviridae family and human parainfluenza virus type 3 of the Paramyxoviridae family. Genes affecting different stages of VSV infection, such as entry/uncoating, gene expression, and assembly/release, were identified. Depletion of the proteins of the coatomer complex I or its upstream effectors ARF1 or GBF1 led to detection of reduced levels of VSV RNA. Coatomer complex I was also required for infection of lymphocytic choriomeningitis virus and human parainfluenza virus type 3. These results highlight the evolutionarily conserved requirements for gene expression of diverse families of NS RNA viruses and demonstrate the involvement of host cell secretory pathway in the process. |
| Used Model | HeLa cells |
| DOI | 10.1073/pnas.1113643108 |
