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Virus Details


VHFID5162

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name HRAS
HF Protein Name GTPase HRas
HF Function Essential for virus replication
Uniprot ID P01112
Protein Sequence View Fasta Sequence
NCBI Gene ID 3265
Host Factor (HF) Name in Paper HRAS
Gene synonyms HRAS1
Ensemble Gene ID ENSG00000174775
Ensemble Transcript ENST00000311189 [P01112-1];ENST00000397594 [P01112-2];ENST00000397596 [P01112-1];ENST00000417302 [P01112-2];ENST00000451590 [P01112-1];ENST00000493230 [P01112-2];ENST00000610977 [P01112-1];ENST00000615062 [P01112-1];ENST00000616241 [P01112-2];ENST00000631404 [P01112-1];ENST00000631967 [P01112-2];ENST00000634098 [P01112-2]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000139, GO:0000165, GO:0001934, GO:0002223, GO:0003924, GO:0005525, GO:0005634, GO:0005737, GO:0005794, GO:0005829, GO:0005886, GO:0006897, GO:0006935, GO:0007050, GO:0007093, GO:0007165, GO:0007166, GO:0007173, GO:0007265, GO:0007411, GO:0008022, GO:0008283, GO:0008284, GO:0008285, GO:0009887, GO:0010629, GO:0019003, GO:0030335, GO:0032729, GO:0034260, GO:0035900, GO:0038095, GO:0038128, GO:0042088, GO:0042832, GO:0043406, GO:0043410, GO:0043524, GO:0043547, GO:0045740, GO:0045944, GO:0046330, GO:0046579, GO:0048013, GO:0048169, GO:0048471, GO:0050679, GO:0050852, GO:0050900, GO:0051291, GO:0070374, GO:0071480, GO:0090303, GO:0090398, GO:0097193, GO:1900029, GO:2000251, GO:2000630,
MINT ID P01112
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 109800
PANTHER ID PTHR24070
PDB ID(s) 121P, 1AA9, 1AGP, 1BKD, 1CLU, 1CRP, 1CRQ, 1CRR, 1CTQ, 1GNP, 1GNQ, 1GNR, 1HE8, 1IAQ, 1IOZ, 1JAH, 1JAI, 1K8R, 1LF0, 1LF5, 1LFD, 1NVU, 1NVV, 1NVW, 1NVX, 1P2S, 1P2T, 1P2U, 1P2V, 1PLJ, 1PLK, 1PLL, 1Q21, 1QRA, 1RVD, 1WQ1, 1XCM, 1XD2, 1XJ0, 1ZVQ, 1ZW6, 221P, 2C5L, 2CE2, 2CL0, 2CL6, 2CL7, 2CLC, 2CLD, 2EVW, 2GDP, 2LCF, 2LWI, 2N42, 2N46, 2Q21, 2QUZ, 2RGA, 2RGB, 2RGC, 2RGD, 2RGE, 2RGG, 2UZI, 2VH5, 2X1V, 3DDC, 3I3S, 3K8Y, 3K9L, 3K9N, 3KKM, 3KKN, 3KUD, 3L8Y, 3L8Z, 3LBH, 3LBI, 3LBN, 3LO5, 3OIU, 3OIV, 3OIW, 3RRY, 3RRZ, 3RS0, 3RS2, 3RS3, 3RS4, 3RS5, 3RS7, 3RSL, 3RSO, 3TGP, 421P, 4DLR, 4DLS, 4DLT, 4DLU, 4DLV, 4DLW, 4DLX, 4DLY, 4DLZ, 4DST, 4DSU, 4EFL, 4EFM, 4EFN, 4G0N, 4G3X, 4K81, 4L9S, 4L9W, 4NYI, 4NYJ, 4NYM, 4Q21, 4RSG, 4URU, 4URV, 4URW, 4URX, 4URY, 4URZ, 4US0, 4US1, 4US2, 4XVQ, 4XVR, 521P, 5B2Z, 5B30, 5E95, 5P21, 5VBE, 5VBZ, 5WDO, 5WDP, 5WDQ, 5WPL, 5X9S, 621P, 6AMB, 6AXG, 6Q21, 721P, 821P,
pfam ID PF00071,
Drug Bank ID DB04315, DB04137, DB03226,
ChEMBL ID CHEMBL2167
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Swine flue virus
Virus Short Name H1N1
Order Unassigned
Virus Family Orthomyxoviridae
Virus Subfamily N.A.
Genus Influenzavirus A
Species Influenza A virus
Host Aquatic birds, human, pig, horse, seals
Cell Tropism Epithelial respiratory cells
Associated Disease Acute febrile respiratory tract infection
Mode of Transmission Respiratory and by animal contact (in human), fecal-oral route from contaminated water (in birds )
VIPR DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Orthomyxoviridae
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/209/orthomyxoviridae
Virus Host DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Orthomyxoviridae

Publication Information

Paper Title Pooled RNAi screen identifies ubiquitin ligase Itch as crucial for influenza A virus release from the endosome during virus entry
Author's Name Wen-Chi Su, Yung-Chia Chen, Chung-Hsin Tseng, Paul Wei-Che Hsu, Kuo-Feng Tung, King-Song Jeng and Michael M. C. Lai
Journal Name PNAS
Pubmed ID 24101521
Abstract Influenza viruses, like other viruses, rely on host factors to support their life cycle as viral proteins usually "hijack," or collaborate with, cellular proteins to execute their functions. Identification and understanding of these factors can increase the knowledge of molecular mechanisms manipulated by the viruses and facilitate development of antiviral drugs. To this end, we developed a unique genome-wide pooled shRNA screen to search for cellular factors important for influenza A virus (IAV) replication. We identified an E3 ubiquitin ligase, Itch, as an essential factor for an early step in the viral life cycle. In Itch knockdown cells, the incorporation of viral ribonucleoprotein complex into endosomes was normal, but its subsequent release from endosomes and transport to the nucleus was retarded. In addition, upon virus infection, Itch was phosphorylated and recruited to the endosomes, where virus particles were located. Furthermore, Itch interacted with viral M1 protein and ubiquitinated M1 protein. Collectively, our findings unravel a critical role of Itch in mediating IAV release from the endosome and offer insights into the mechanism for IAV uncoating during virus entry. These findings also highlight the feasibility of pooled RNAi screening for exploring the cellular cofactors of lytic viruses.
Used Model A549,MDCK and HEK293T cells
DOI 10.1073/pnas.1312374110