| Gene Name | HSPA4 |
| HF Protein Name | Heat shock 70 kDa protein 4 |
| HF Function | Positively regulates viral genome replication |
| Uniprot ID | P34932 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 3308 |
| Host Factor (HF) Name in Paper | Hsp70 |
| Gene synonyms | APG2 |
| Ensemble Gene ID | ENSG00000170606 |
| Ensemble Transcript | ENST00000304858 [P34932-1] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0005524, GO:0005829, GO:0006986, GO:0045040, GO:0051131, GO:0070062, |
| MINT ID | P34932 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 601113 |
| PANTHER ID | PTHR19375 |
| PDB ID(s) | N.A., |
| pfam ID | PF00012, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Japanese encephalitis virus |
| Virus Short Name | JEV |
| Order | Unassigned |
| Virus Family | Flaviviridae |
| Virus Subfamily | N.A. |
| Genus | Flavivirus |
| Species | Japanese encephalitis virus |
| Host | Human, mammals, mosquitoes and ticks |
| Cell Tropism | N.A. |
| Associated Disease | Hemorrhagic fever, encephalitis |
| Mode of Transmission | Arthropod bite, mainly mosquitoes |
| VIPR DB link | http://www.viprbrc.org/brc/home.spg?decorator=flavi |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/flaviviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Flaviviridae |
| Paper Title | Heat shock protein 70 is associated with replicase complex of Japanese encephalitis virus and positively regulates viral genome replication |
| Author's Name | Jing Ye, Zheng Chen, Bo Zhang, Huan Miao, Ali Zohaib, Qiuping Xu, Huanchun Chen and Shengbo Cao |
| Journal Name | PLOS One |
| Pubmed ID | 24086464 |
| Abstract | Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes the most prevalent viral encephalitis in Asia. The NS5 protein of JEV is a key component of the viral replicase complex, which plays a crucial role in viral pathogenesis. In this study, tandem affinity purification (TAP) followed by mass spectrometry analysis was performed to identify novel host proteins that interact with NS5. Heat shock protein 70 (Hsp70), eukaryotic elongation factor 1-alpha (eEF-1alpha) and ras-related nuclear protein (Ran) were demonstrated to interact with NS5. In addition to NS5, Hsp70 was also found to interact with NS3 which is another important member of the replicase complex. It was observed that the cytoplasmic Hsp70 partially colocalizes with the components of viral replicase complex including NS3, NS5 and viral dsRNA during JEV infection. Knockdown of Hsp70 resulted in a significantly reduced JEV genome replication. Further analysis reveals that Hsp70 enhances the stability of viral proteins in JEV replicase complex. These results suggest an important role for Hsp70 in regulating JEV replication, which provides a potential target for the development of anti-JEV therapies. |
| Used Model | HEK293T and BHK-21 cells |
| DOI | 10.1371/journal.pone.0075188 |
