| Gene Name | MED12 |
| HF Protein Name | Mediator of RNA polymerase II transcription subunit 12 |
| HF Function | Activates interferon-beta after virus infection |
| Uniprot ID | Q93074 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 9968 |
| Host Factor (HF) Name in Paper | MED12 |
| Gene synonyms | ARC240 CAGH45 HOPA KIAA0192 TNRC11 TRAP230 |
| Ensemble Gene ID | ENSG00000184634 |
| Ensemble Transcript | ENST00000374080 [Q93074-1];ENST00000374102 [Q93074-3] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000151, GO:0000980, GO:0001104, GO:0001105, GO:0001843, GO:0003682, GO:0003712, GO:0003713, GO:0004872, GO:0005634, GO:0005654, GO:0006367, GO:0007492, GO:0007507, GO:0008013, GO:0008022, GO:0014003, GO:0014044, GO:0016020, GO:0016592, GO:0019827, GO:0019904, GO:0021510, GO:0030178, GO:0030374, GO:0030518, GO:0030521, GO:0036342, GO:0042809, GO:0045893, GO:0045944, GO:0046966, GO:0048702, GO:0060070, GO:0060071, GO:0061630, GO:0090245, GO:1990403, |
| MINT ID | Q93074 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 300188 |
| PANTHER ID | N.A. |
| PDB ID(s) | N.A., |
| pfam ID | PF09497, PF12145, PF12144, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Sendai virus |
| Virus Short Name | SeV |
| Order | Mononegavirales |
| Virus Family | Paramyxoviridae |
| Virus Subfamily | N.A. |
| Genus | Respirovirus |
| Species | Murine respirovirus |
| Host | Mammals |
| Cell Tropism | N.A. |
| Associated Disease | Acute febrile respiratory tract infection |
| Mode of Transmission | Respiratory |
| VIPR DB link | http://www.viprbrc.org/brc/vipr_allSpecies_search.do?method=SubmitForm&decorator=paramyxo |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/199/paramyxoviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Paramyxoviridae |
| Paper Title | Genome-wide RNAi screen reveals a new role of a WNT/ CTNNB1 signaling pathway as negative regulator of virus-induced innate immune responses |
| Author's Name | Martin Baril, Salwa Es-Saad, Laurent Chatel-Chaix, Karin Fink, Tram Pham, Vale rie-Ann Raymond, Karine Audette, Anne-Sophie Guenier, Jean Duchaine, Marc Servant, Marc Bilodeau, E ric Cohen, Nathalie Grandvaux, Daniel Lamarre |
| Journal Name | PLOS Pathogens |
| Pubmed ID | 23785285 |
| Abstract | To identify new regulators of antiviral innate immunity, we completed the first genome-wide gene silencing screen assessing the transcriptional response at the interferon-beta (IFNB1) promoter following Sendai virus (SeV) infection. We now report a novel link between WNT signaling pathway and the modulation of retinoic acid-inducible gene I (RIG-I)-like receptor (RLR)-dependent innate immune responses. Here we show that secretion of WNT2B and WNT9B and stabilization of beta-catenin (CTNNB1) upon virus infection negatively regulate expression of representative inducible genes IFNB1, IFIT1 and TNF in a CTNNB1-dependent effector mechanism. The antiviral response is drastically reduced by glycogen synthase kinase 3 (GSK3) inhibitors but restored in CTNNB1 knockdown cells. The findings confirm a novel regulation of antiviral innate immunity by a canonical-like WNT/CTNNB1 signaling pathway. The study identifies novel avenues for broad-spectrum antiviral targets and preventing immune-mediated diseases upon viral infection. |
| Used Model | HEK 293T and A549 cells |
| DOI | 10.1371/journal.ppat.1003416 |
