| Gene Name | POLR2D |
| HF Protein Name | DNA-directed RNA polymerase II subunit RPB4 |
| HF Function | Activates interferon-beta after virus infection |
| Uniprot ID | O15514 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 5433 |
| Host Factor (HF) Name in Paper | POLR2D |
| Gene synonyms | N.A. |
| Ensemble Gene ID | ENSG00000144231 |
| Ensemble Transcript | ENST00000272645 |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000166, GO:0000288, GO:0000398, GO:0000932, GO:0003899, GO:0005634, GO:0005654, GO:0005665, GO:0005829, GO:0006283, GO:0006351, GO:0006366, GO:0006367, GO:0006368, GO:0006370, GO:0008543, GO:0016070, GO:0016607, GO:0031369, GO:0031990, GO:0034402, GO:0035019, GO:0042795, GO:0045948, GO:0050434, GO:0060964, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 606017 |
| PANTHER ID | N.A. |
| PDB ID(s) | 2C35, 5IY6, 5IY7, 5IY8, 5IY9, 5IYA, 5IYB, 5IYC, 5IYD, |
| pfam ID | PF03874, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Sendai virus |
| Virus Short Name | SeV |
| Order | Mononegavirales |
| Virus Family | Paramyxoviridae |
| Virus Subfamily | N.A. |
| Genus | Respirovirus |
| Species | Murine respirovirus |
| Host | Mammals |
| Cell Tropism | N.A. |
| Associated Disease | Acute febrile respiratory tract infection |
| Mode of Transmission | Respiratory |
| VIPR DB link | http://www.viprbrc.org/brc/vipr_allSpecies_search.do?method=SubmitForm&decorator=paramyxo |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/199/paramyxoviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Paramyxoviridae |
| Paper Title | Genome-wide RNAi screen reveals a new role of a WNT/ CTNNB1 signaling pathway as negative regulator of virus-induced innate immune responses |
| Author's Name | Martin Baril, Salwa Es-Saad, Laurent Chatel-Chaix, Karin Fink, Tram Pham, Vale rie-Ann Raymond, Karine Audette, Anne-Sophie Guenier, Jean Duchaine, Marc Servant, Marc Bilodeau, E ric Cohen, Nathalie Grandvaux, Daniel Lamarre |
| Journal Name | PLOS Pathogens |
| Pubmed ID | 23785285 |
| Abstract | To identify new regulators of antiviral innate immunity, we completed the first genome-wide gene silencing screen assessing the transcriptional response at the interferon-beta (IFNB1) promoter following Sendai virus (SeV) infection. We now report a novel link between WNT signaling pathway and the modulation of retinoic acid-inducible gene I (RIG-I)-like receptor (RLR)-dependent innate immune responses. Here we show that secretion of WNT2B and WNT9B and stabilization of beta-catenin (CTNNB1) upon virus infection negatively regulate expression of representative inducible genes IFNB1, IFIT1 and TNF in a CTNNB1-dependent effector mechanism. The antiviral response is drastically reduced by glycogen synthase kinase 3 (GSK3) inhibitors but restored in CTNNB1 knockdown cells. The findings confirm a novel regulation of antiviral innate immunity by a canonical-like WNT/CTNNB1 signaling pathway. The study identifies novel avenues for broad-spectrum antiviral targets and preventing immune-mediated diseases upon viral infection. |
| Used Model | HEK 293T and A549 cells |
| DOI | 10.1371/journal.ppat.1003416 |
