| Gene Name | EIF2AK2 |
| HF Protein Name | Eukaryotic translation initiation factor 2 alpha kinase 2 |
| HF Function | Essential for virus replication |
| Uniprot ID | F1NLD7 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 395147 |
| Host Factor (HF) Name in Paper | EIF2AK2 |
| Gene synonyms | N.A. |
| Ensemble Gene ID | ENSGALG00000010560 |
| Ensemble Transcript | ENSGALT00000017183 |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000186, GO:0003725, GO:0004694, GO:0005524, GO:0005829, GO:0006412, GO:0009615, GO:0009636, GO:0017148, GO:0030968, GO:0032722, GO:0032874, GO:0033689, GO:0034198, GO:0035455, GO:0042802, GO:0043066, GO:0045071, GO:0046777, GO:0048471, GO:0051092, GO:1900225, GO:1901224, GO:1902033, GO:1902036, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | N.A. |
| PANTHER ID | PTHR11042:SF102 |
| PDB ID(s) | N.A., |
| pfam ID | PF00035, PF00069, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Gallus gallus (Chicken) |
| Virus Name | Newcastle disease virus |
| Virus Short Name | NDV |
| Order | Mononegavirales |
| Virus Family | Paramyxoviridae |
| Virus Subfamily | N.A. |
| Genus | Avulavirus |
| Species | Newcastle disease virus |
| Host | Birds |
| Cell Tropism | N.A. |
| Associated Disease | Variable fatal disease |
| Mode of Transmission | Direct contact with secretions from the nose, mouth, and eyes |
| VIPR DB link | http://www.viprbrc.org/brc/vipr_allSpecies_search.do?method=SubmitForm&decorator=paramyxo |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/199/paramyxoviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Paramyxoviridae |
| Paper Title | Activation of the PKR/eIF2alpha signaling cascade inhibits replication of Newcastle disease virus |
| Author's Name | Shilei Zhang, Yingjie Sun, Hongjun Chen, Yabin Dai, Yuan Zhan, Shengqing Yu, Xusheng Qiu, Lei Tan, Cuiping Song and Chan Ding |
| Journal Name | Virology Journal |
| Pubmed ID | 24684861 |
| Abstract | BACKGROUND: Newcastle Disease virus (NDV) causes severe and economically significant disease in almost all birds. However, factors that affect NDV replication in host cells are poorly understood. NDV generates long double-stranded RNA (dsRNA) molecules during transcription of single-stranded genomic RNA. Protein kinase R (PKR) is activated by dsRNA. The aim of this study was to elucidate the role of PKR in NDV infection. RESULTS: NDV infection led to the activation of dsRNA-dependent PKR and phosphorylation of its substrate, translation initiation factor eIF2alpha, in a dose-dependent manner by either the lentogenic strain LaSota or a velogenic strain Herts/33. PKR activation coincided with the accumulation of dsRNA induced by NDV infection. PKR knockdown remarkably decreased eIF2alpha phosphorylation as well as IFN-beta mRNA levels, leading to the augmentation of extracellular virus titer. Furthermore, siRNA knockdown or phosphorylation of eIF2alpha or okadaic acid treatment significantly impaired NDV replication, indicating the critical role of the PKR/eIF2alpha signaling cascade in NDV infection. CONCLUSION: PKR is activated by dsRNA generated by NDV infection and inhibits NDV replication by eIF2alpha phosphorylation. This study provides insight into NDV-host interactions for the development of candidate antiviral strategies. |
| Used Model | HeLa cells |
| DOI | 10.1186/1743-422X-11-62 |
