| Gene Name | HYOU1 |
| HF Protein Name | Hypoxia up-regulated protein 1 |
| HF Function | Promotes endoplasmic reticulum to cytosol membrane penetration of the nonenveloped virus Simian Virus 40 |
| Uniprot ID | Q9Y4L1 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 10525 |
| Host Factor (HF) Name in Paper | Grp170 |
| Gene synonyms | GRP170 ORP150 |
| Ensemble Gene ID | ENSG00000149428 |
| Ensemble Transcript | ENST00000617285 [Q9Y4L1-1];ENST00000630669 [Q9Y4L1-1] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0002931, GO:0005524, GO:0005576, GO:0005783, GO:0005788, GO:0005790, GO:0005925, GO:0006888, GO:0006898, GO:0016020, GO:0034663, GO:0034976, GO:0036498, GO:0051087, GO:0070062, GO:0071456, GO:0071682, GO:1903298, GO:1903382, |
| MINT ID | Q9Y4L1 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 601746 |
| PANTHER ID | PTHR19375 |
| PDB ID(s) | N.A., |
| pfam ID | PF00012, |
| Drug Bank ID | N.A., |
| ChEMBL ID | CHEMBL2216741 |
| Organism | Homo sapiens (Human) |
| Virus Name | Simian Virus 40 |
| Virus Short Name | SV40 |
| Order | Unassigned |
| Virus Family | Polyomaviridae |
| Virus Subfamily | N.A. |
| Genus | Betapolyomavirus |
| Species | Simian virus 40 |
| Host | Mammals, human |
| Cell Tropism | N.A. |
| Associated Disease | N.A. |
| Mode of Transmission | N.A. |
| VIPR DB link | N.A. |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/129/polyomaviridae |
| Virus Host DB link | N.A. |
| Paper Title | A nucleotide exchange factor promotes endoplasmic reticulum-to-cytosol membrane penetrationof the nonenveloped virus simian virus 40 |
| Author's Name | Takamasa Inoue, Billy Tsai |
| Journal Name | Journal Of Virology |
| Pubmed ID | 25653441 |
| Abstract | The nonenveloped simian polyomavirus (PyV) simian virus 40 (SV40) hijacks the endoplasmic reticulum (ER) quality control machinery to penetrate the ER membrane and reach the cytosol, a critical infection step. During entry, SV40 traffics to the ER, where host-induced conformational changes render the virus hydrophobic. The hydrophobic virus binds and integrates into the ER lipid bilayer to initiate membrane penetration. However, prior to membrane transport, the hydrophobic SV40 recruits the ER-resident Hsp70 BiP, which holds the virus in a transport-competent state until it is ready to cross the ER membrane. Here we probed how BiP disengages from SV40 to enable the virus to penetrate the ER membrane. We found that nucleotide exchange factor (NEF) Grp170 induces nucleotide exchange of BiP and releases SV40 from BiP. Importantly, this reaction promotes SV40 ER-to-cytosol transport and infection. The human BK PyV also relies on Grp170 for successful infection. Interestingly, SV40 mobilizes a pool of Grp170 into discrete puncta in the ER called foci. These foci, postulated to represent the ER membrane penetration site, harbor ER components, including BiP, known to facilitate viral ER-to-cytosol transport. Our results thus identify a nucleotide exchange activity essential for catalyzing the most proximal event before ER membrane penetration of PyVs. |
| Used Model | COS-7 and HEK 293 cells |
| DOI | 10.1128/JVI.03552-14 |
