Virus Details


VHFID7398

Host Factor Information

Gene Name TSPAN9
HF Protein Name Tetraspanin-9
HF Function Host proteins required for SINV Entry
Uniprot ID O75954
Protein Sequence View Fasta Sequence
NCBI Gene ID 10867
Host Factor (HF) Name in Paper TSPAN9
Gene synonyms NET5
Ensemble Gene ID ENSG00000011105
Ensemble Transcript ENST00000011898;ENST00000537971
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0005886, GO:0005887, GO:0005925, GO:0007166, GO:0097197,
MINT ID O75954
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 613137
PANTHER ID N.A.
PDB ID(s) N.A.,
pfam ID PF00335,
Drug Bank ID N.A.,
ChEMBL ID N.A.
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Sindbis virus
Virus Short Name SINV
Order Unassigned
Virus Family Togaviridae
Virus Subfamily N.A.
Genus Alphavirus
Species Sindbis virus
Host Human, mammals,mosquitoes and birds
Cell Tropism N.A.
Associated Disease Sindbis fever
Mode of Transmission By infected mosquito
VIPR DB link https://www.viprbrc.org/brc/vipr_allSpecies_search.spg?method=SubmitForm&decorator=toga
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/275/togaviridae
Virus Host DB link N.A.

Publication Information

Paper Title Genome Wide RNAi screen identifies novel host proteins required for alphavirus entry
Author's Name Yaw Shin Ooi, Katie M. Stiles, Catherine Y. Liu, Gwen M. Taylor and Margaret Kielian
Journal Name PLoS Pathogens
Pubmed ID 24367265
Abstract The enveloped alphaviruses include important and emerging human pathogens such as Chikungunya virus and Eastern equine encephalitis virus. Alphaviruses enter cells by clathrin-mediated endocytosis, and exit by budding from the plasma membrane. While there has been considerable progress in defining the structure and function of the viral proteins, relatively little is known about the host factors involved in alphavirus infection. We used a genome-wide siRNA screen to identify host factors that promote or inhibit alphavirus infection in human cells. Fuzzy homologue (FUZ), a protein with reported roles in planar cell polarity and cilia biogenesis, was required for the clathrin-dependent internalization of both alphaviruses and the classical endocytic ligand transferrin. The tetraspanin membrane protein TSPAN9 was critical for the efficient fusion of low pH-triggered virus with the endosome membrane. FUZ and TSPAN9 were broadly required for infection by the alphaviruses Sindbis virus, Semliki Forest virus, and Chikungunya virus, but were not required by the structurally-related flavivirus Dengue virus. Our results highlight the unanticipated functions of FUZ and TSPAN9 in distinct steps of alphavirus entry and suggest novel host proteins that may serve as targets for antiviral therapy.
Used Model U-2 OS
DOI 10.1371/journal.ppat.1003835