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Virus Details


VHFID7465

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name FMR1
HF Protein Name Synaptic functional regulator FMR1
HF Function Interacts with the 3? untranslated region of virus genomic RNA
Uniprot ID Q06787
Protein Sequence View Fasta Sequence
NCBI Gene ID 2332
Host Factor (HF) Name in Paper FMR1
Gene synonyms N.A.
Ensemble Gene ID ENSG00000102081
Ensemble Transcript ENST00000218200 [Q06787-9];ENST00000370470 [Q06787-6];ENST00000370471 [Q06787-10];ENST00000370475 [Q06787-1];ENST00000440235 [Q06787-8]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000381, GO:0000775, GO:0002092, GO:0002151, GO:0003682, GO:0003723, GO:0003729, GO:0003730, GO:0005634, GO:0005654, GO:0005694, GO:0005730, GO:0005737, GO:0005829, GO:0005844, GO:0005845, GO:0006397, GO:0006974, GO:0007215, GO:0007399, GO:0008017, GO:0008266, GO:0008380, GO:0010369, GO:0010494, GO:0014069, GO:0015030, GO:0016020, GO:0016032, GO:0019034, GO:0019897, GO:0030054, GO:0030371, GO:0030424, GO:0030425, GO:0030426, GO:0030529, GO:0031047, GO:0031369, GO:0032433, GO:0033129, GO:0033592, GO:0034046, GO:0034644, GO:0035064, GO:0035197, GO:0035198, GO:0035613, GO:0036464, GO:0042734, GO:0042802, GO:0042803, GO:0042995, GO:0043005, GO:0043022, GO:0043197, GO:0043204, GO:0043488, GO:0043679, GO:0044325, GO:0044830, GO:0045202, GO:0045211, GO:0045727, GO:0045947, GO:0046928, GO:0046982, GO:0048027, GO:0048471, GO:0051028, GO:0051489, GO:0051491, GO:0060964, GO:0060998, GO:0060999, GO:0070840, GO:0071598, GO:0072711, GO:0097386, GO:0098586, GO:0098793, GO:0098794, GO:0098908, GO:1,
MINT ID Q06787
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 300623
PANTHER ID N.A.
PDB ID(s) 2BKD, 2FMR, 2LA5, 2QND, 4OVA, 4QVZ, 4QW2, 5DE5, 5DE8, 5DEA, 5UWJ, 5UWO,
pfam ID PF05641, PF16098, PF12235, PF00013,
Drug Bank ID N.A.,
ChEMBL ID N.A.
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Tick-borne encephalitis virus
Virus Short Name TBEV
Order Unassigned
Virus Family Flaviviridae
Virus Subfamily N.A.
Genus Flavivirus
Species Tick-borne encephalitis virus
Host Human, mammals, mosquitoes and ticks
Cell Tropism N.A.
Associated Disease Meningitis and encephalitis
Mode of Transmission Tick bite,from an infected mother to fetus
VIPR DB link http://www.viprbrc.org/brc/home.spg?decorator=flavi
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/flaviviridae
Virus Host DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Flaviviridae

Publication Information

Paper Title Identification and analysis of host proteins that interact with the 3?- untranslated region of tick-borne encephalitis virus genomic RNA
Author's Name Memi Mutoa, Wataru Kamitanib, Mizuki Sakaia, Minato Hiranoa, Shintaro Kobayashia, Hiroaki Kariwaa, Kentaro Yoshii
Journal Name Virus Research
Pubmed ID 29545014
Abstract Tick-borne encephalitis virus (TBEV) causes severe neurological disease, but the pathogenetic mechanism is unclear. The conformational structure of the 3-untranslated region (UTR) of TBEV is associated with its virulence. We tried to identify host proteins interacting with the 3-UTR of TBEV. Cellular proteins of HEK293T cells were co-precipitated with biotinylated RNAs of the 3-UTR of low- and high-virulence TBEV strains and subjected to mass spectrometry analysis. Fifteen host proteins were found to bind to the 3-UTR of TBEV, four of which-cold shock domain containing-E1 (CSDE1), spermatid perinuclear RNA binding protein (STRBP), fragile X mental retardation protein (FMRP), and interleukin enhancer binding factor 3 (ILF3)-bound specifically to that of the low-virulence strain. An RNA immunoprecipitation and pull-down assay confirmed the interactions of the complete 3-UTRs of TBEV genomic RNA with CSDE1, FMRP, and ILF3. Partial deletion of the stem loop (SL) 3 to SL 5 structure of the variable region of the 3-UTR did not affect interactions with the host proteins, but the interactions were markedly suppressed by deletion of the complete SL 3, 4, and 5 structures, as in the high-virulence TBEV strain. Further analysis of the roles of host proteins in the neurologic pathogenicity of TBEV is warranted.
Used Model HEK293T cells
DOI 10.1016/j.virusres.2018.03.006