| Gene Name | DDX21 |
| HF Protein Name | Nucleolar RNA helicase 2 |
| HF Function | Interacts with the 3? untranslated region of virus genomic RNA |
| Uniprot ID | Q9NR30 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 9188 |
| Host Factor (HF) Name in Paper | DDX21 |
| Gene synonyms | N.A. |
| Ensemble Gene ID | ENSG00000165732 |
| Ensemble Transcript | ENST00000354185 [Q9NR30-1];ENST00000620315 [Q9NR30-2] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0001649, GO:0003723, GO:0003725, GO:0004004, GO:0005524, GO:0005634, GO:0005654, GO:0005730, GO:0005737, GO:0006364, GO:0006366, GO:0009615, GO:0010501, GO:0016020, GO:0019843, GO:0030515, GO:0035198, GO:0043330, GO:0045815, GO:0097322, |
| MINT ID | Q9NR30 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 606357 |
| PANTHER ID | N.A. |
| PDB ID(s) | 2M3D, |
| pfam ID | PF00270, PF08152, PF00271, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Tick-borne encephalitis virus |
| Virus Short Name | TBEV |
| Order | Unassigned |
| Virus Family | Flaviviridae |
| Virus Subfamily | N.A. |
| Genus | Flavivirus |
| Species | Tick-borne encephalitis virus |
| Host | Human, mammals, mosquitoes and ticks |
| Cell Tropism | N.A. |
| Associated Disease | Meningitis and encephalitis |
| Mode of Transmission | Tick bite,from an infected mother to fetus |
| VIPR DB link | http://www.viprbrc.org/brc/home.spg?decorator=flavi |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/flaviviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Flaviviridae |
| Paper Title | Identification and analysis of host proteins that interact with the 3?- untranslated region of tick-borne encephalitis virus genomic RNA |
| Author's Name | Memi Mutoa, Wataru Kamitanib, Mizuki Sakaia, Minato Hiranoa, Shintaro Kobayashia, Hiroaki Kariwaa, Kentaro Yoshii |
| Journal Name | Virus Research |
| Pubmed ID | 29545014 |
| Abstract | Tick-borne encephalitis virus (TBEV) causes severe neurological disease, but the pathogenetic mechanism is unclear. The conformational structure of the 3-untranslated region (UTR) of TBEV is associated with its virulence. We tried to identify host proteins interacting with the 3-UTR of TBEV. Cellular proteins of HEK293T cells were co-precipitated with biotinylated RNAs of the 3-UTR of low- and high-virulence TBEV strains and subjected to mass spectrometry analysis. Fifteen host proteins were found to bind to the 3-UTR of TBEV, four of which-cold shock domain containing-E1 (CSDE1), spermatid perinuclear RNA binding protein (STRBP), fragile X mental retardation protein (FMRP), and interleukin enhancer binding factor 3 (ILF3)-bound specifically to that of the low-virulence strain. An RNA immunoprecipitation and pull-down assay confirmed the interactions of the complete 3-UTRs of TBEV genomic RNA with CSDE1, FMRP, and ILF3. Partial deletion of the stem loop (SL) 3 to SL 5 structure of the variable region of the 3-UTR did not affect interactions with the host proteins, but the interactions were markedly suppressed by deletion of the complete SL 3, 4, and 5 structures, as in the high-virulence TBEV strain. Further analysis of the roles of host proteins in the neurologic pathogenicity of TBEV is warranted. |
| Used Model | HEK293T cells |
| DOI | 10.1016/j.virusres.2018.03.006 |
