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Virus Details


VHFID7468

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name HSPA1A
HF Protein Name Heat shock 70 kDa protein 1A
HF Function Interacts with the 3? untranslated region of virus genomic RNA
Uniprot ID P0DMV8
Protein Sequence View Fasta Sequence
NCBI Gene ID 3303;3304
Host Factor (HF) Name in Paper HSPA1A
Gene synonyms HSP72 HSPA1 HSX70
Ensemble Gene ID ENSG00000204389
Ensemble Transcript ENST00000375651 [P0DMV8-1];ENST00000400040;ENST00000430065 [P0DMV8-1];ENST00000433487 [P0DMV8-1];ENST00000441618 [P0DMV8-1]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0001106, GO:0001618, GO:0001664, GO:0003723, GO:0005102, GO:0005524, GO:0005576, GO:0005634, GO:0005654, GO:0005737, GO:0005739, GO:0005783, GO:0005813, GO:0005814, GO:0005829, GO:0005925, GO:0006402, GO:0006986, GO:0008285, GO:0010628, GO:0016234, GO:0016235, GO:0016607, GO:0016887, GO:0019899, GO:0030308, GO:0030512, GO:0030529, GO:0031072, GO:0031249, GO:0031396, GO:0031397, GO:0031625, GO:0031982, GO:0032436, GO:0032757, GO:0032991, GO:0034599, GO:0034605, GO:0042026, GO:0042623, GO:0042826, GO:0043066, GO:0043312, GO:0043488, GO:0044183, GO:0045296, GO:0045648, GO:0046034, GO:0047485, GO:0048471, GO:0050821, GO:0051082, GO:0051092, GO:0051131, GO:0055131, GO:0060548, GO:0070062, GO:0070370, GO:0070434, GO:0072562, GO:0090063, GO:0090084, GO:0097201, GO:0097718, GO:1900034, GO:1901029, GO:1901673, GO:1902236, GO:1902380, GO:1903265, GO:1904722, GO:1904813, GO:2001240,
MINT ID N.A.
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 140550
PANTHER ID PTHR19375
PDB ID(s) 1HJO, 1S3X, 1XQS, 2E88, 2E8A, 2LMG, 3A8Y, 3ATU, 3ATV, 3AY9, 3D2E, 3D2F, 3JXU, 3LOF, 3Q49, 4IO8, 4J8F, 4PO2, 4WV5, 4WV7, 5AQW, 5AQX, 5AQY, 5AQZ, 5AR0, 5BN8, 5BN9, 5BPL, 5BPM, 5BPN, 5GJJ, 5MKR, 5MKS,
pfam ID PF00012,
Drug Bank ID N.A.,
ChEMBL ID CHEMBL5460
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Tick-borne encephalitis virus
Virus Short Name TBEV
Order Unassigned
Virus Family Flaviviridae
Virus Subfamily N.A.
Genus Flavivirus
Species Tick-borne encephalitis virus
Host Human, mammals, mosquitoes and ticks
Cell Tropism N.A.
Associated Disease Meningitis and encephalitis
Mode of Transmission Tick bite,from an infected mother to fetus
VIPR DB link http://www.viprbrc.org/brc/home.spg?decorator=flavi
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/flaviviridae
Virus Host DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Flaviviridae

Publication Information

Paper Title Identification and analysis of host proteins that interact with the 3?- untranslated region of tick-borne encephalitis virus genomic RNA
Author's Name Memi Mutoa, Wataru Kamitanib, Mizuki Sakaia, Minato Hiranoa, Shintaro Kobayashia, Hiroaki Kariwaa, Kentaro Yoshii
Journal Name Virus Research
Pubmed ID 29545014
Abstract Tick-borne encephalitis virus (TBEV) causes severe neurological disease, but the pathogenetic mechanism is unclear. The conformational structure of the 3-untranslated region (UTR) of TBEV is associated with its virulence. We tried to identify host proteins interacting with the 3-UTR of TBEV. Cellular proteins of HEK293T cells were co-precipitated with biotinylated RNAs of the 3-UTR of low- and high-virulence TBEV strains and subjected to mass spectrometry analysis. Fifteen host proteins were found to bind to the 3-UTR of TBEV, four of which-cold shock domain containing-E1 (CSDE1), spermatid perinuclear RNA binding protein (STRBP), fragile X mental retardation protein (FMRP), and interleukin enhancer binding factor 3 (ILF3)-bound specifically to that of the low-virulence strain. An RNA immunoprecipitation and pull-down assay confirmed the interactions of the complete 3-UTRs of TBEV genomic RNA with CSDE1, FMRP, and ILF3. Partial deletion of the stem loop (SL) 3 to SL 5 structure of the variable region of the 3-UTR did not affect interactions with the host proteins, but the interactions were markedly suppressed by deletion of the complete SL 3, 4, and 5 structures, as in the high-virulence TBEV strain. Further analysis of the roles of host proteins in the neurologic pathogenicity of TBEV is warranted.
Used Model HEK293T cells
DOI 10.1016/j.virusres.2018.03.006