| Gene Name | WNK4 |
| HF Protein Name | Serine/threonine-protein kinase WNK4 |
| HF Function | Essential for viral DNA replication |
| Uniprot ID | Q96J92 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 65266 |
| Host Factor (HF) Name in Paper | WNK4 |
| Gene synonyms | PRKWNK4 |
| Ensemble Gene ID | N.A. |
| Ensemble Transcript | ENST00000246914 [Q96J92-1] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0004674, GO:0005524, GO:0005829, GO:0005923, GO:0006468, GO:0006811, GO:0006821, GO:0008104, GO:0010766, GO:0016020, GO:0019869, GO:0035556, GO:0050794, GO:0050801, GO:0070294, GO:0072156, GO:0090188, |
| MINT ID | Q96J92 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 601844 |
| PANTHER ID | N.A. |
| PDB ID(s) | 2V3S, 4CH9, 4CHB, |
| pfam ID | PF12202, PF00069, |
| Drug Bank ID | N.A., |
| ChEMBL ID | CHEMBL1795196 |
| Organism | Homo sapiens (Human) |
| Virus Name | Vaccinia Virus |
| Virus Short Name | VACV |
| Order | Unassigned |
| Virus Family | Poxviridae |
| Virus Subfamily | Chordopoxvirinae |
| Genus | Orthopoxvirus |
| Species | Vaccinia virus |
| Host | Human, mammals |
| Cell Tropism | Dendritic cells, monocytes/macrophages, b lymphocytes, activated t lymphocytes |
| Associated Disease | N.A. |
| Mode of Transmission | N.A. |
| VIPR DB link | https://www.viprbrc.org/brc/home.spg?decorator=pox |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/74/poxviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Poxviridae |
| Paper Title | RNAi sreening reveals proteasome and cullin3 dependent stages in vaccinia virus infection |
| Author's Name | Jason Mercer, Berend Snijder, Raphael Sacher, Christine Burkard, Christopher Karl Ernst Bleck, Henning Stahlberg, Lucas Pelkmans, and Ari Helenius |
| Journal Name | Cell Reports |
| Pubmed ID | 23084750 |
| Abstract | A two-step, automated, high-throughput RNAi silencing screen was used to identify host cell factors required during vaccinia virus infection. Validation and analysis of clustered hits revealed previously unknown processes during virus entry, including a mechanism for genome uncoating. Viral core proteins were found to be already ubiquitinated during virus assembly. After entering the cytosol of an uninfected cell, the viral DNA was released from the core through the activity of the cells proteasomes. Next, a Cullin3-based ubiquitin ligase mediated a further round of ubiquitination and proteasome action. This was needed in order to initiate viral DNA replication. The results accentuate the value of large-scale RNAi screens in providing directions for detailed cell biological investigation of complex pathways. The list of cell functions required during poxvirus infection will, moreover, provide a resource for future virus-host cell interaction studies and for the discovery of antivirals. |
| Used Model | HeLa cells |
| DOI | 10.1016/j.celrep.2012.09.003 |
