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Virus Details


VHFID7592

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name POLA1
HF Protein Name DNA polymerase alpha catalytic subunit
HF Function Essential for viral DNA replication
Uniprot ID P09884
Protein Sequence View Fasta Sequence
NCBI Gene ID 5422
Host Factor (HF) Name in Paper POLA1
Gene synonyms POLA
Ensemble Gene ID ENSG00000101868
Ensemble Transcript ENST00000379059
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000082, GO:0000083, GO:0000166, GO:0000731, GO:0001882, GO:0003677, GO:0003682, GO:0003887, GO:0005634, GO:0005635, GO:0005654, GO:0005658, GO:0005730, GO:0005829, GO:0006260, GO:0006269, GO:0006270, GO:0006271, GO:0006272, GO:0006273, GO:0006281, GO:0006303, GO:0008283, GO:0016032, GO:0016363, GO:0019901, GO:0032201, GO:0046872, GO:0051539,
MINT ID P09884
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 301220
PANTHER ID N.A.
PDB ID(s) 1K0P, 1K18, 1N5G, 4Q5V, 4QCL, 4Y97, 5EXR, 5IUD, 6AS7,
pfam ID PF12254, PF00136, PF03104, PF08996,
Drug Bank ID DB00242, DB00631, DB01073, DB01280,
ChEMBL ID CHEMBL1828
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Vaccinia Virus
Virus Short Name VACV
Order Unassigned
Virus Family Poxviridae
Virus Subfamily Chordopoxvirinae
Genus Orthopoxvirus
Species Vaccinia virus
Host Human, mammals
Cell Tropism Dendritic cells, monocytes/macrophages, b lymphocytes, activated t lymphocytes
Associated Disease N.A.
Mode of Transmission N.A.
VIPR DB link https://www.viprbrc.org/brc/home.spg?decorator=pox
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/74/poxviridae
Virus Host DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Poxviridae

Publication Information

Paper Title RNAi sreening reveals proteasome and cullin3 dependent stages in vaccinia virus infection
Author's Name Jason Mercer, Berend Snijder, Raphael Sacher, Christine Burkard, Christopher Karl Ernst Bleck, Henning Stahlberg, Lucas Pelkmans, and Ari Helenius
Journal Name Cell Reports
Pubmed ID 23084750
Abstract A two-step, automated, high-throughput RNAi silencing screen was used to identify host cell factors required during vaccinia virus infection. Validation and analysis of clustered hits revealed previously unknown processes during virus entry, including a mechanism for genome uncoating. Viral core proteins were found to be already ubiquitinated during virus assembly. After entering the cytosol of an uninfected cell, the viral DNA was released from the core through the activity of the cells proteasomes. Next, a Cullin3-based ubiquitin ligase mediated a further round of ubiquitination and proteasome action. This was needed in order to initiate viral DNA replication. The results accentuate the value of large-scale RNAi screens in providing directions for detailed cell biological investigation of complex pathways. The list of cell functions required during poxvirus infection will, moreover, provide a resource for future virus-host cell interaction studies and for the discovery of antivirals.
Used Model HeLa cells
DOI 10.1016/j.celrep.2012.09.003