Hostfactor - VHFIDB

Gene Name	P2RY1
HF Protein Name	P2Y purinoceptor 1
HF Function	Essential for viral DNA replication
Uniprot ID	P47900
Protein Sequence	View Fasta Sequence
NCBI Gene ID	5028
Host Factor (HF) Name in Paper	P2RY1
Gene synonyms	N.A.
Ensemble Gene ID	ENSG00000169860
Ensemble Transcript	ENST00000305097
KEGG ID	Go to KEGG Database
Gene Ontology ID(s)	GO:0001934, GO:0004872, GO:0005524, GO:0005739, GO:0005886, GO:0005887, GO:0007166, GO:0007186, GO:0007193, GO:0007200, GO:0007204, GO:0007568, GO:0008347, GO:0009612, GO:0009986, GO:0010469, GO:0010700, GO:0014069, GO:0016323, GO:0016324, GO:0019233, GO:0023019, GO:0030168, GO:0030425, GO:0031686, GO:0032962, GO:0042755, GO:0043270, GO:0043531, GO:0044297, GO:0045028, GO:0045031, GO:0045032, GO:0045211, GO:0045944, GO:0046887, GO:0046982, GO:0051100, GO:0060406, GO:0070374, GO:0070848, GO:0071407, GO:0072659, GO:0090075, GO:0097110, GO:0097746,
MINT ID	P47900
STRING	Click to see interaction map
GWAS Analysis	Click to see gwas analysis
OMIM ID	601167
PANTHER ID	PTHR24231:SF2
PDB ID(s)	1Y36, 4XNV, 4XNW,
pfam ID	PF00001,
Drug Bank ID	N.A.,
ChEMBL ID	CHEMBL4315
Organism	Homo sapiens (Human)

Virus Name	Vaccinia Virus
Virus Short Name	VACV
Order	Unassigned
Virus Family	Poxviridae
Virus Subfamily	Chordopoxvirinae
Genus	Orthopoxvirus
Species	Vaccinia virus
Host	Human, mammals
Cell Tropism	Dendritic cells, monocytes/macrophages, b lymphocytes, activated t lymphocytes
Associated Disease	N.A.
Mode of Transmission	N.A.
VIPR DB link	https://www.viprbrc.org/brc/home.spg?decorator=pox
ICTV DB link	https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/74/poxviridae
Virus Host DB link	http://www.genome.jp/virushostdb/view/?virus_lineage=Poxviridae

Paper Title	RNAi sreening reveals proteasome and cullin3 dependent stages in vaccinia virus infection
Author's Name	Jason Mercer, Berend Snijder, Raphael Sacher, Christine Burkard, Christopher Karl Ernst Bleck, Henning Stahlberg, Lucas Pelkmans, and Ari Helenius
Journal Name	Cell Reports
Pubmed ID	23084750
Abstract	A two-step, automated, high-throughput RNAi silencing screen was used to identify host cell factorsÂ required during vaccinia virus infection. Validation and analysis of clustered hits revealed previously unknown processes during virus entry, including a mechanism for genome uncoating. Viral core proteins were found to be already ubiquitinatedÂ during virus assembly. After entering the cytosol of an uninfected cell, the viral DNA was released from the core through the activity of the cells proteasomes. Next, a Cullin3-based ubiquitin ligase mediated a further round of ubiquitination and proteasome action. This was needed in order to initiate viral DNA replication. The results accentuate the value of large-scale RNAi screens in providing directions for detailed cell biological investigation of complex pathways. The list of cell functions required during poxvirus infection will, moreover, provide a resource for future virus-host cell interaction studies and for the discovery of antivirals.
Used Model	HeLa cells
DOI	10.1016/j.celrep.2012.09.003

Virus Details