| Gene Name | FHOD1 |
| HF Protein Name | FH1/FH2 domain-containing protein 1 |
| HF Function | Promotes Vaccinia virus actin?based motility |
| Uniprot ID | Q9Y613 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 29109 |
| Host Factor (HF) Name in Paper | FHOD1 |
| Gene synonyms | FHOS FHOS1 |
| Ensemble Gene ID | ENSG00000135723 |
| Ensemble Transcript | ENST00000258201 |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0003779, GO:0005634, GO:0005737, GO:0005829, GO:0005856, GO:0007097, GO:0014704, GO:0016020, GO:0019904, GO:0032059, GO:0042802, GO:0043621, GO:0045944, GO:0051492, GO:0051496, GO:0051660, |
| MINT ID | Q9Y613 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 606881 |
| PANTHER ID | PTHR23213:SF189 |
| PDB ID(s) | 3DAD, |
| pfam ID | PF02181, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Vaccinia Virus |
| Virus Short Name | VACV |
| Order | Unassigned |
| Virus Family | Poxviridae |
| Virus Subfamily | Chordopoxvirinae |
| Genus | Orthopoxvirus |
| Species | Vaccinia virus |
| Host | Human, mammals |
| Cell Tropism | Dendritic cells, monocytes/macrophages, b lymphocytes, activated t lymphocytes |
| Associated Disease | N.A. |
| Mode of Transmission | N.A. |
| VIPR DB link | https://www.viprbrc.org/brc/home.spg?decorator=pox |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/74/poxviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Poxviridae |
| Paper Title | The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin?based motility |
| Author's Name | Diego E. Alvarez and Herve Agaisse |
| Journal Name | Journal Of Cell Biology |
| Pubmed ID | 24062339 |
| Abstract | Vaccinia virus dissemination relies on the N-WASP-ARP2/3 pathway, which mediates actin tail formation underneath cell-associated extracellular viruses (CEVs). Here, we uncover a previously unappreciated role for the formin FHOD1 and the small GTPase Rac1 in vaccinia actin tail formation. FHOD1 depletion decreased the number of CEVs forming actin tails and impaired the elongation rate of the formed actin tails. Recruitment of FHOD1 to actin tails relied on its GTPase binding domain in addition to its FH2 domain. In agreement with previous studies showing that FHOD1 is activated by the small GTPase Rac1, Rac1 was enriched and activated at the membrane surrounding actin tails. Rac1 depletion or expression of dominant-negative Rac1 phenocopied the effects of FHOD1 depletion and impaired the recruitment of FHOD1 to actin tails. FHOD1 overexpression rescued the actin tail formation defects observed in cells overexpressing dominant-negative Rac1. Altogether, our results indicate that, to display robust actin-based motility, vaccinia virus integrates the activity of the N-WASP-ARP2/3 and Rac1-FHOD1 pathways. |
| Used Model | HeLa S3 and BS-C-1 cells |
| DOI | 10.1083/jcb.201303055 |
