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Virus Details


VHFID7674

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name RAC1
HF Protein Name Ras-related C3 botulinum toxin substrate 1
HF Function Promotes Vaccinia virus actin?based motility
Uniprot ID P63000
Protein Sequence View Fasta Sequence
NCBI Gene ID 5879
Host Factor (HF) Name in Paper Rac1
Gene synonyms TC25
Ensemble Gene ID ENSG00000136238
Ensemble Transcript ENST00000348035 [P63000-1];ENST00000356142 [P63000-2]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000139, GO:0001934, GO:0002551, GO:0003924, GO:0004674, GO:0005525, GO:0005737, GO:0005789, GO:0005802, GO:0005829, GO:0005886, GO:0005925, GO:0006954, GO:0007155, GO:0007160, GO:0007186, GO:0007596, GO:0008283, GO:0008361, GO:0009611, GO:0009653, GO:0010310, GO:0010591, GO:0010592, GO:0010811, GO:0016020, GO:0016601, GO:0017137, GO:0019899, GO:0019901, GO:0030027, GO:0030032, GO:0030036, GO:0030041, GO:0030168, GO:0030334, GO:0030667, GO:0031116, GO:0031295, GO:0031529, GO:0031996, GO:0032587, GO:0032707, GO:0034446, GO:0035025, GO:0035556, GO:0036464, GO:0038095, GO:0038096, GO:0042470, GO:0042826, GO:0043065, GO:0043197, GO:0043312, GO:0045453, GO:0045740, GO:0048010, GO:0048012, GO:0048013, GO:0048261, GO:0048870, GO:0050690, GO:0051022, GO:0051056, GO:0051117, GO:0051492, GO:0051496, GO:0051668, GO:0051894, GO:0055038, GO:0060071, GO:0060263, GO:0070062, GO:0071260, GO:0071526, GO:0090023, GO:0097178, GO:0101003, GO:1900026,
MINT ID P63000
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 602048
PANTHER ID N.A.
PDB ID(s) 1E96, 1FOE, 1G4U, 1HE1, 1HH4, 1I4D, 1I4L, 1I4T, 1MH1, 1RYF, 1RYH, 2FJU, 2H7V, 2NZ8, 2P2L, 2RMK, 2VRW, 2WKP, 2WKQ, 2WKR, 2YIN, 3B13, 3BJI, 3RYT, 3SBD, 3SBE, 3SU8, 3SUA, 3TH5, 4GZL, 4GZM, 4YON, 5FI0, 5HZH, 5N6O, 5O33, 6BC1,
pfam ID PF00071,
Drug Bank ID DB00514, DB04315,
ChEMBL ID CHEMBL6094
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Vaccinia Virus
Virus Short Name VACV
Order Unassigned
Virus Family Poxviridae
Virus Subfamily Chordopoxvirinae
Genus Orthopoxvirus
Species Vaccinia virus
Host Human, mammals
Cell Tropism Dendritic cells, monocytes/macrophages, b lymphocytes, activated t lymphocytes
Associated Disease N.A.
Mode of Transmission N.A.
VIPR DB link https://www.viprbrc.org/brc/home.spg?decorator=pox
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/74/poxviridae
Virus Host DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Poxviridae

Publication Information

Paper Title The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin?based motility
Author's Name Diego E. Alvarez and Herve Agaisse
Journal Name Journal Of Cell Biology
Pubmed ID 24062339
Abstract Vaccinia virus dissemination relies on the N-WASP-ARP2/3 pathway, which mediates actin tail formation underneath cell-associated extracellular viruses (CEVs). Here, we uncover a previously unappreciated role for the formin FHOD1 and the small GTPase Rac1 in vaccinia actin tail formation. FHOD1 depletion decreased the number of CEVs forming actin tails and impaired the elongation rate of the formed actin tails. Recruitment of FHOD1 to actin tails relied on its GTPase binding domain in addition to its FH2 domain. In agreement with previous studies showing that FHOD1 is activated by the small GTPase Rac1, Rac1 was enriched and activated at the membrane surrounding actin tails. Rac1 depletion or expression of dominant-negative Rac1 phenocopied the effects of FHOD1 depletion and impaired the recruitment of FHOD1 to actin tails. FHOD1 overexpression rescued the actin tail formation defects observed in cells overexpressing dominant-negative Rac1. Altogether, our results indicate that, to display robust actin-based motility, vaccinia virus integrates the activity of the N-WASP-ARP2/3 and Rac1-FHOD1 pathways.
Used Model HeLa S3 and BS-C-1 cells
DOI 10.1083/jcb.201303055