| Gene Name | SAMD9 |
| HF Protein Name | Sterile alpha motif domain-containing protein 9 |
| HF Function | Restriction Factors |
| Uniprot ID | Q5K651 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 54809 |
| Host Factor (HF) Name in Paper | SAMD9 |
| Gene synonyms | C7orf5 DRIF1 KIAA2004 OEF1 |
| Ensemble Gene ID | ENSG00000205413 |
| Ensemble Transcript | ENST00000379958;ENST00000620985 |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0005737, GO:0005769, GO:0005829, GO:0034058, GO:0043231, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 610455 |
| PANTHER ID | N.A. |
| PDB ID(s) | N.A., |
| pfam ID | PF07647, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Vaccinia Virus |
| Virus Short Name | VACV |
| Order | Unassigned |
| Virus Family | Poxviridae |
| Virus Subfamily | Chordopoxvirinae |
| Genus | Orthopoxvirus |
| Species | Vaccinia virus |
| Host | Human, mammals |
| Cell Tropism | Dendritic cells, monocytes/macrophages, b lymphocytes, activated t lymphocytes |
| Associated Disease | N.A. |
| Mode of Transmission | N.A. |
| VIPR DB link | https://www.viprbrc.org/brc/home.spg?decorator=pox |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/dsdna-viruses-2011/w/dsdna_viruses/74/poxviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Poxviridae |
| Paper Title | Identification of restriction factors by human genome wide RNA interference screening of viral host range mutants exemplified by discovery of SAMD9 and WDR6 as inhibitors of the vaccinia virus K1L-C7L- mutant |
| Author's Name | Gilad Sivan, Pinar Ormanoglu, Eugen C. Buehler, Scott E. Martin, Bernard Mossa |
| Journal Name | mBio (American Society Of Microbiology) |
| Pubmed ID | 26242627 |
| Abstract | RNA interference (RNAi) screens intended to identify host factors that restrict virus replication may fail if the virus already counteracts host defense mechanisms. To overcome this limitation, we are investigating the use of viral host range mutants that exhibit impaired replication in nonpermissive cells. A vaccinia virus (VACV) mutant with a deletion of both the C7L and K1L genes, K1L(-)C7L(-), which abrogates replication in human cells at a step prior to late gene expression, was chosen for this strategy. We carried out a human genome-wide small interfering RNA (siRNA) screen in HeLa cells infected with a VACV K1L(-)C7L(-) mutant that expresses the green fluorescent protein regulated by a late promoter. This positive-selection screen had remarkably low background levels and resulted in the identification of a few cellular genes, notably SAMD9 and WDR6, from approximately 20,000 tested that dramatically enhanced green fluorescent protein expression. Replication of the mutant virus was enabled by multiple siRNAs to SAMD9 or WDR6. Moreover, SAMD9 and WDR6 clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 knockout HeLa cell lines were permissive for replication of the K1L(-)C7L(-) mutant, in agreement with the siRNA data. Expression of exogenous SAMD9 or interferon regulatory factor 1 restricted replication of the K1L(-)C7L(-) mutant in the SAMD9(-/-) cells. Independent interactions of SAMD9 with the K1 and C7 proteins were suggested by immunoprecipitation. Knockout of WDR6 did not reduce the levels of SAMD9 and interactions of WDR6 with SAMD9, C7, and K1 proteins were not detected, suggesting that these restriction factors act independently but possibly in the same innate defense pathway. |
| Used Model | HeLa cells |
| DOI | 10.1128/mBio.01122-15 |
