Virus Details


VHFID8622

Host Factor Information

Gene Name AP2A2
HF Protein Name AP-2 complex subunit alpha-2
HF Function Essential for VEEV infection
Uniprot ID O94973
Protein Sequence View Fasta Sequence
NCBI Gene ID 161
Host Factor (HF) Name in Paper AP2A2
Gene synonyms ADTAB CLAPA2 HIP9 HYPJ KIAA0899
Ensemble Gene ID ENSG00000183020
Ensemble Transcript ENST00000332231 [O94973-2];ENST00000448903 [O94973-1];ENST00000528815 [O94973-3]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0005829, GO:0005886, GO:0006886, GO:0007018, GO:0008289, GO:0019886, GO:0019901, GO:0030122, GO:0030666, GO:0030667, GO:0030669, GO:0031410, GO:0032802, GO:0034383, GO:0035615, GO:0036020, GO:0043312, GO:0045334, GO:0048013, GO:0050690, GO:0060071, GO:0061024, GO:0072583, GO:0097718, GO:0101003,
MINT ID O94973
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 607242
PANTHER ID N.A.
PDB ID(s) N.A.,
pfam ID PF01602, PF02296, PF02883,
Drug Bank ID N.A.,
ChEMBL ID N.A.
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Venezuelan equine encephalitis
Virus Short Name VEEV
Order Unassigned
Virus Family Togaviridae
Virus Subfamily N.A.
Genus Alphavirus
Species Venezuelan equine encephalitis
Host Human, mammals,mosquitoes and birds
Cell Tropism N.A.
Associated Disease Venezuelan equine encephalitis or encephalomyelitis (vee )
Mode of Transmission By infected mosquito
VIPR DB link https://www.viprbrc.org/brc/vipr_allSpecies_search.spg?method=SubmitForm&decorator=toga
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/275/togaviridae
Virus Host DB link N.A.

Publication Information

Paper Title siRNA Screen Identifies Trafficking Host Factors that Modulate Alphavirus Infection
Author's Name Sheli R. Radoshitzky, Gianluca Pegoraro, Xiaoli Chi, Lian Dong, Chih-Yuan Chiang, Lucas Jozwick, Jeremiah C. Clester, Christopher L. Cooper, Duane Courier, David P. Langan, Knashka Underwood, Kathleen A. Kuehl, Mei G. Sun, Yi?ngyun Cai, Shuiqing Yu, Robin Burk, Rouzbeh Zamani, Krishna Kota, Jens H. Kuhn, Sina Bavari
Journal Name PLoS Pathogens
Pubmed ID 27031835
Abstract Little is known about the repertoire of cellular factors involved in the replication of pathogenic alphaviruses. To uncover molecular regulators of alphavirus infection, and to identify candidate drug targets, we performed a high-content imaging-based siRNA screen. We revealed an actin-remodeling pathway involving Rac1, PIP5K1- alpha, and Arp3, as essential for infection by pathogenic alphaviruses. Infection causes cellular actin rearrangements into large bundles of actin filaments termed actin foci. Actin foci are generated late in infection concomitantly with alphavirus envelope (E2) expression and are dependent on the activities of Rac1 and Arp3. E2 associates with actin in alphavirus-infected cells and co-localizes with Rac1-PIP5K1-alpha along actin filaments in the context of actin foci. Finally, Rac1, Arp3, and actin polymerization inhibitors interfere with E2 trafficking from the trans-Golgi network to the cell surface, suggesting a plausible model in which transport of E2 to the cell surface is mediated via Rac1- and Arp3-dependent actin remodeling.
Used Model HeLa cells
DOI 10.1371/journal.ppat.1005466