Download Help

Virus Details


VHFID8637

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name PDCD6IP
HF Protein Name Programmed cell death 6-interacting protein
HF Function Essential for VEEV infection
Uniprot ID Q8WUM4
Protein Sequence View Fasta Sequence
NCBI Gene ID 10015
Host Factor (HF) Name in Paper PDCD6IP
Gene synonyms AIP1 ALIX KIAA1375
Ensemble Gene ID ENSG00000170248
Ensemble Transcript ENST00000307296 [Q8WUM4-1];ENST00000457054 [Q8WUM4-2]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000281, GO:0000915, GO:0000920, GO:0001772, GO:0005815, GO:0005829, GO:0005923, GO:0005925, GO:0006915, GO:0010824, GO:0015031, GO:0016020, GO:0019058, GO:0031871, GO:0036258, GO:0039702, GO:0042470, GO:0042641, GO:0042803, GO:0043209, GO:0045199, GO:0046755, GO:0048306, GO:0051260, GO:0070062, GO:0070830, GO:0070971, GO:0090543, GO:0090559, GO:0090611, GO:1903543, GO:1903551, GO:1903553, GO:1903561,
MINT ID Q8WUM4
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 608074
PANTHER ID N.A.
PDB ID(s) 2OEV, 2OEW, 2OEX, 2OJQ, 2R02, 2R03, 2R05, 2XS1, 2XS8, 2ZNE, 3C3O, 3C3Q, 3C3R, 3E1R, 3WUV, 4JJY,
pfam ID PF13949, PF03097,
Drug Bank ID N.A.,
ChEMBL ID N.A.
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Venezuelan equine encephalitis
Virus Short Name VEEV
Order Unassigned
Virus Family Togaviridae
Virus Subfamily N.A.
Genus Alphavirus
Species Venezuelan equine encephalitis
Host Human, mammals,mosquitoes and birds
Cell Tropism N.A.
Associated Disease Venezuelan equine encephalitis or encephalomyelitis (vee )
Mode of Transmission By infected mosquito
VIPR DB link https://www.viprbrc.org/brc/vipr_allSpecies_search.spg?method=SubmitForm&decorator=toga
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/275/togaviridae
Virus Host DB link N.A.

Publication Information

Paper Title siRNA Screen Identifies Trafficking Host Factors that Modulate Alphavirus Infection
Author's Name Sheli R. Radoshitzky, Gianluca Pegoraro, Xiaoli Chi, Lian Dong, Chih-Yuan Chiang, Lucas Jozwick, Jeremiah C. Clester, Christopher L. Cooper, Duane Courier, David P. Langan, Knashka Underwood, Kathleen A. Kuehl, Mei G. Sun, Yi?ngyun Cai, Shuiqing Yu, Robin Burk, Rouzbeh Zamani, Krishna Kota, Jens H. Kuhn, Sina Bavari
Journal Name PLoS Pathogens
Pubmed ID 27031835
Abstract Little is known about the repertoire of cellular factors involved in the replication of pathogenic alphaviruses. To uncover molecular regulators of alphavirus infection, and to identify candidate drug targets, we performed a high-content imaging-based siRNA screen. We revealed an actin-remodeling pathway involving Rac1, PIP5K1- alpha, and Arp3, as essential for infection by pathogenic alphaviruses. Infection causes cellular actin rearrangements into large bundles of actin filaments termed actin foci. Actin foci are generated late in infection concomitantly with alphavirus envelope (E2) expression and are dependent on the activities of Rac1 and Arp3. E2 associates with actin in alphavirus-infected cells and co-localizes with Rac1-PIP5K1-alpha along actin filaments in the context of actin foci. Finally, Rac1, Arp3, and actin polymerization inhibitors interfere with E2 trafficking from the trans-Golgi network to the cell surface, suggesting a plausible model in which transport of E2 to the cell surface is mediated via Rac1- and Arp3-dependent actin remodeling.
Used Model HeLa cells
DOI 10.1371/journal.ppat.1005466